A Strain of Pseudomonas sp. Isolated from Piggery Wastewater Treatment Systems with Heterotrophic Nitrification Capability in Taiwan
A high concentration of NH4+ in piggery wastewater is major problem in Taiwan. Therefore, in our study, we isolated native heterotrophic nitrifiers for piggery wastewater treatment. Heterotrophic nitrifier AS-1 was isolated and characterized from the activated sludge of a piggery wastewater system. Sets of triplicate crimp-sealed serum bottles were used to demonstrate the heterotrophic nitrifying capability of strain AS-1 in an incubator at 30°C. All serum bottles contained 80 mL medium, and the remainder of the bottle headspace was filled with pure oxygen. The experimental results showed that 2.5 ± 0.2 mmol L−1 NH4+ was removed by 58 hours, and, eventually, 1.5 ± 0.5 mmol L−1 N2 and 0.2 ± 0.0 mmol L−1 N2O were produced. The removal rate of NH4+ by the strain AS-1 was 1.75 mmol NH4+ g cell−1 h−1. This strain was then identified as Pseudomonas alcaligenes (97% identity) by sequencing its 16S rDNA and comparing it with other microorganisms. Thus, strain AS-1 displays high promise for future application for in situ NH4+ removal from piggery wastewater.
The investigators thank the National Science Council, Executive Yuan of the Republic of China, Taiwan, for financially supporting this research under Contract No. NSC 91−2317-B-059-004. E. D. R. Brierley, Institute of Water and Environment, Cranfield University, UK, is appreciated for providing valuable reference papers and advice. In adition, R. H. Chen is appreciated for helping with portions of the experiments.
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