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Current Microbiology

, Volume 53, Issue 2, pp 135–140 | Cite as

Purification and Characterization of an Alginate Lyase from Marine Bacterium Vibrio sp. Mutant Strain 510-64

  • Xiaoke Hu
  • Xiaolu Jiang
  • Huey-min Hwang
Article

Abstract

Marine Vibrio sp. 510 was chosen as a parent strain for screening high producers of alginate lyase using the complex mutagenesis of Ethyl Methanesulphonate and UV radiation treatments. The mutant strain Vibrio sp. 510-64 was selected and its alginate lyase activity was increased by 3.87-fold (reaching 46.12 EU/mg) over that of the parent strain. An extracellular alginate lyase was purified from Vibrio sp. 510-64 cultural supernatant by successive fractionation on DEAE Sepharose FF and two steps of Superdex 75. The purified enzyme yielded a single band on SDS-PAGE with the molecular weight of 34.6 kDa. Data of the N-terminal amino acid sequence indicated that this protein might be a novel alginate lyase. The substrate specificity results demonstrated that the alginate lyase had the specificity for poly G block.

Keywords

Alginate Vibrio Lyase Alginate Lyase Ethyl Methanesulphonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a grant from “The Shandong Province Key Project of China” (Project No. 003110112).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Institute of Marine Drug and Food, Ocean University of ChinaQingdaoP.R. China
  2. 2.Department of BiologyJackson State UniversityJacksonU.S.A.

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