Evaluation of Herpes Simplex Detection in Corneal Scrapings by Three Molecular Methods
- 132 Downloads
Herpes simplex virus (HSV)-1 keratitis (HSK) is a sight-threatening ocular infection with worldwide occurrence. A prompt laboratory diagnosis is often very useful. The purpose of this study was to evaluate molecular methods as rapid diagnostic tools compared with cell culture of HSK. Corneal scrapings from patients with clinically suspected HSK were tested by direct immunofluorescence assay (IFA) for HSV-1 antigen and by polymerase chain reaction (PCR) for HSV-1 DNA, and an attempt for viral isolation was performed on Vero cell line culture. Positive samples by cell culture were 20.8%, whereas PCR was positive in 29.2%, and IFA was positive in 33.3%. IFA had better sensitivity (80%) and negative predictive value (81.8%) than PCR (70% and 76.9%, respectively); however, PCR had better specificity (71.4%) and positive predictive value (63.6%). This indicates that a combination of cell culture, IFA and PCR constitutes the best set of tools for diagnosis of clinically suspected cases of HSK. Documented infection can be further assessed by cell-culture technique or PCR depending laboratory availability.
- 8.Khodadoost MA, Sabahi F, Behroz MJ, Roustai MH, Saderi H, Amini-Bavil-Olyaee S, et al. (2004). Study of a polymerase chain reaction based method for detection of herpes simplex virus 1 DNA among Iranian patients with ocular herpetic keratitis infection. Jpn J Ophthalmol 48:328–332CrossRefPubMedGoogle Scholar
- 11.Schmidt NJ (1979). Cell culture techniques for diagnostic virology. In: Lennette EH, Schmidt NJ (eds) Diagnostic procedures for viral, richettsial and chlamydial infections. Washington, DC: American Public Health Association, pp 65–139Google Scholar
- 12.Landry ML, Hsuing GD (2001) Primary isolation of viruses. In: Specter S, Hodinka RL, Young SA (eds) Clinical virology manual, 3rd ed, ASM Press, Washington, pp 27–42Google Scholar
- 13.Johnson G, Nelson S, Petric M, Tellier R (2000). Comprehensive PCR-based assay for detection and species identification of human herpes viruses. J Clin Microb 38:3274–3279Google Scholar
- 19.Chan EL, Brandt K, Horsman GB (2001). Comparison of chemicon simulfluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and direct immunofluorescence staining for detection of varicella zoster virus. Clin Diagn Lab Immunol 8:909–912CrossRefPubMedGoogle Scholar