Current Microbiology

, Volume 52, Issue 2, pp 81–85 | Cite as

Differential Predation by Bdellovibrio bacteriovorus 109J

  • Amy M. Rogosky
  • Pamela L. Moak
  • Elizabeth A. B. Emmert
Article

Abstract

Bdellovibrio bacteriovorus is a predatory bacterium that can replicate only inside Gram-negative bacteria. We incubated B. bacteriovorus 109J in a mixture of two prey cells present in equal numbers and enumerated prey cells after 3 h of predation. In multiple prey pairings, B. bacteriovorus preferentially lysed on one prey over the other. When prey were individually incubated with B. bacteriovorus, they were preyed on with different efficiencies. Three prey had only 5–8% of cells remaining after Bdellovibrio predation and the other three prey had 37–43% of cells remaining. Timing of attachment of B. bacteriovorus to prey cells also varied with Bdellovibrio attachment to more preferred prey occurring the fastest. These results suggest that B. bacteriovorus 109J does not randomly infect prey cells but infects and kills some prey more readily than others.

Literature Cited

  1. 1.
    Drutz DJ (1976) Response of Neisseria gonorrhoeae to Bdellovibrio species. Infect Immun 13:247–251PubMedGoogle Scholar
  2. 2.
    Fratamico PM, Whiting RC (1995) Ability of Bdellovibrio bacteriovorus 109J to lyse gram-negative food-borne pathogenic and spoilage bacteria. J Food Prot 58:160–164Google Scholar
  3. 3.
    Germinda JJ, (1987) Isolation of Bdellovibrio spp. that prey on Azospirillum brasilense in soil. Can J Microbiol 33:459–461Google Scholar
  4. 4.
    Jurkevitch E (2000) The genus Bdellovibrio. In: Dworkin M, Flakow S, Rosenberg E, Schleifer KH, Stackenbrandt E (eds) The prokaryotes. New York: Springer-Verlag. http://www.link.springer-ny. com/link/service/books/10125/ Google Scholar
  5. 5.
    Jurkevitch E, Minz D, Ramati B, Barel G (2000) Prey range characterization, ribotyping, and diversity of soil and rhizosphere Bdellovibrio spp. isolated on phytopathogenic bacteria. Appl Environ Microbiol 66:2365–2371CrossRefPubMedGoogle Scholar
  6. 6.
    Koval SF, Bayer ME (1997) Bacterial capsules: no barrier against Bdellovibrio. Microbiol 143:749–753PubMedGoogle Scholar
  7. 7.
    Koval SF, Hynes SH (1991) Effect of paracrystalline protein surface layers on predation by Bdellovibrio bacteriovorus. J Bacteriol 173:2244–2249PubMedGoogle Scholar
  8. 8.
    Lambert C, Smith MCM, Sockett RE (2003) A novel assay to monitor predator-prey interactions for Bdellovibrio bacteriovorus 109J reveals a role for methyl-accepting chemotaxis proteins in predation. Environ Microbiol 5:127–132CrossRefPubMedGoogle Scholar
  9. 9.
    Pineiro SA, Sahaniuk GE, Romberg E, Williams HN (2004) Predation pattern and phylogenetic analysis of Bdellovibrionaceae from the Great Salt Lake, Utah. Curr Microbiol 48:113–117PubMedGoogle Scholar
  10. 10.
    Rendulic S, Jagtap P, Rosinus A, Eppinger M, Baar C, Lanz C, Keller H, Lambert C, Evans KJ, Goesmann A, Meyer F, Sockett RE, Schuster SC (2004) A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective. Science 303:689–692CrossRefPubMedGoogle Scholar
  11. 11.
    Ruby EG (1991) The genus Bdellovibrio. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes. 2nd ed. New York: Springer-Verlag, pp 3400–3415Google Scholar
  12. 12.
    Schoeffield AJ, Williams HN (1990) Efficiencies of recovery of bdellovibrios from brackish-water environments by using various bacterial species as prey. Appl Environ Microbiol 56:230–236PubMedGoogle Scholar
  13. 13.
    Varon M, Shilo M (1969) Attachment of Bdellovibrio bacteriovorus to cell wall mutants of Salmonella spp. and Escherichia coli. J Bacteriol 97:977–979PubMedGoogle Scholar
  14. 14.
    Varon M, Shilo M (1969) Interaction of Bdellovibrio bacteriovorus and host bacteria. II. Intracellular growth and development of Bdellovibrio bacteriovorus in liquid cultures. J Bacteriol 99:136–141PubMedGoogle Scholar
  15. 15.
    Varon M, (1981) Interaction of Bdellovibrio with its prey in mixed microbial populations. Microb Ecol 7:97–105Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Amy M. Rogosky
    • 1
    • 3
  • Pamela L. Moak
    • 2
    • 4
  • Elizabeth A. B. Emmert
    • 2
  1. 1.Department of BiologyWest Virginia Wesleyan CollegeBuckhannonUSA
  2. 2.Department of Biological SciencesSalisbury UniversitySalisburyUSA
  3. 3.Microbilogy, Immunology, and Molecular Genetics DepartmentUniversity of KentuckyLexington
  4. 4.Department of Biological SciencesVirginia Polytechnic Institute and State UniversityBlacksburg

Personalised recommendations