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Current Microbiology

, Volume 51, Issue 4, pp 267–269 | Cite as

Diagnosis of Bacteria In Vitro by Mass Spectrometric Fingerprinting:A Pilot Study

  • Matthias Lechner
  • Manfred Fille
  • Johann Hausdorfer
  • Manfred P.  Dierich
  • Josef Rieder
Article

Abstract

The identification of bacteria by using conventional microbiological techniques can be very time-consuming and circumstantial. In contrast, the headspace screening of bacterial cultures by analyzing their emitted volatile compounds using mass spectrometry might provide a novel approach in diagnostic microbiology. In the present study different strains of Escherichia coli, Klebsiella, Citrobacter, Pseudomonas aeruginosa, Staphylococcus aureus, and Helicobacter pylori were investigated. The volatile compounds emitted by these bacteria in vitro were analyzed using proton-transfer-reaction mass spectrometry, which allows rapid and sensitive measurement. The detected patterns of volatile compounds produced by the investigated bacteria were compared and substantial differences regarding both quantity and quality were observed. In conclusion, the present study is the first to describe headspace screening of bacterial cultures as a potential diagnostic approach in medical microbiology.

Keywords

Volatile Organic Compound Volatile Compound Klebsiella Drift Tube Atomic Mass Unit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Matthias Lechner
    • 1
  • Manfred Fille
    • 1
  • Johann Hausdorfer
    • 1
  • Manfred P.  Dierich
    • 1
  • Josef Rieder
    • 2
  1. 1.Department of Hygiene, Microbiology and Social Medicine, Section Hygiene and Medical MicrobiologyMedical University of InnsbruckInnsbruckAustria
  2. 2.Department of Anaesthesiology and Critical Care MedicineUniversity Hospital of InnsbruckInnsbruckAustria

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