Current Microbiology

, Volume 50, Issue 4, pp 202–207

Antibiotic Susceptibility of Lactobacillus and Bifidobacterium Species from the Human Gastrointestinal Tract

  • Susana Delgado
  • Ana Belén Flórez
  • Baltasar Mayo
Article

Abstract

One hundred and twenty-two strains of Bifidobacterium and Lactobacillus species have been tested against 12 antibiotics and two antibiotic mixtures by a commercial system (Sensititre Anaero3; Treck Diagnostic Systems). The upper limits of some minimum inhibitory concentrations (MICs) were completed on MRS agar plates by the NCCLS procedure. All strains were sensitive to chloramphenicol and imipenem and most of the strains were resistant to metronidazole. Bifidobacteria isolates were susceptible to cefoxitin, whereas about half of the lactobacilli were resistant. Approximately 30% of the Bifidobacterium isolates were resistant to tetracycline, as well as five Lactobacillus strains belonging to four different species. None of the tested Bifidobacterium isolates was resistant to vancomycin, whereas a species-dependent resistance was found among the lactobacilli. Single strains of Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Lactobacillus brevis were resistant to erythromycin and/or clindamycin. Most of the observed resistances seemed to be intrinsic, but some others could be compatible with transmissible determinants.

Literature Cited

  1. 1.
    Anonymous (1997) Approved standard M11-A4. Methods for antimicrobial susceptibility testing of anaerobic bacteria, 4th edn. National Committee for Clinical Laboratory Standards, VillanovaGoogle Scholar
  2. 2.
    Axelsson, LT, Ahrné, SE, Andersson, MC, Stahl, SR 1988Identification and cloning of a plasmid-encoded erythromycin resistance determinant from Lactobacillus reuteriPlasmid20171174CrossRefPubMedGoogle Scholar
  3. 3.
    Charteris, WP, Kelly, PM, Morelli, L, Collins, JK 1998Antibiotic susceptibility of potentially probiotic Lactobacillus speciesJ Food Prot6116361643PubMedGoogle Scholar
  4. 4.
    Charteris, WP, Kelly, PM, Morelli, L, Collins, JK 1998Antibiotic susceptibility of potentially probiotic Bifidobacterium isolates from the human gastrointestinal tractLett Appl Microbiol26333337CrossRefPubMedGoogle Scholar
  5. 5.
    Church, DL, Bryant, RD, Sim, V, Laishley, EJ 1996Metronidazole susceptibility and the presence of hydrogenase in pathogenic bacteriaAnaerobe2147153CrossRefGoogle Scholar
  6. 6.
    Condon, S 1983Aerobic metabolism of lactic acid bacteriaIr J Food Sci Technol71525Google Scholar
  7. 7.
    Danielsen, M, Wind, A 2004Susceptibility of Lactobacillus spp. to antimicrobial agentsInt J Food Microbiol82111CrossRefGoogle Scholar
  8. 8.
    Freeman, CD, Klutman, NE, Lamp, KC 1997Metronidazole: A therapeutic review and updateDrugs54679708PubMedGoogle Scholar
  9. 9.
    Gasser, F 1994Safety of lactic acid bacteria and their occurrence in human clinical infectionsBull Inst Pasteur924567Google Scholar
  10. 10.
    Hamilton-Miller, JM, Shah, S 1994Susceptibility patterns of vaginal lactobacilli to eleven oral antibioticsJ Antimicrob Chemother3310591060PubMedGoogle Scholar
  11. 11.
    Handwerger, S, Pucci, MJ, Volk, KJ, Liu, J, Lee, MS 1994Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactateJ Bacteriol176260264PubMedGoogle Scholar
  12. 12.
    Klaenhammer, TR, Muller, MJ 1999Selection and design of probioticsInt J Food Microbiol504557CrossRefPubMedGoogle Scholar
  13. 13.
    Lim, KS, Huh, CS, Baek, YJ 1992Antimicrobial susceptibility of bifidobacteriaJ Dairy Sci7621682174Google Scholar
  14. 14.
    Liu, CF, Fung, ZF, Wu, CL, Chung, TC 1996Molecular characterization of a plasmid-borne (pTC82) chloramphenicol resistance determinant (catTC) from Lactobacillus reuteri G4Plasmid36116124CrossRefPubMedGoogle Scholar
  15. 15.
    Mandar, R, Lijvukene, K, Huftt, P, Karki, T, Mikelsaar, M 2001Antibacterial susceptibility of intestinal lactobacilli of healthy childrenScand J Infect Dis33344349CrossRefPubMedGoogle Scholar
  16. 16.
    Matteuzzi, D, Crocciani, F, Brigidi, P 1983Antimicrobial susceptibility of BifidobacteriumAnn Microbiol Inst Pasteur134A339349CrossRefGoogle Scholar
  17. 17.
    Netherwood, T, Bowden, R, Harrosin, P, O’Donnel, AG, Parker, DS, Gilbert, HJ 1999Gene transfer in the gastrointestinal tractAppl Environ Microbiol6551395141Google Scholar
  18. 18.
    Scott, KP, Melville, CM, Barbosa, TM, Flint, HJ 2000Occurrence of the new tetracycline resistance gene tet(W) in bacteria from the human gutAntimicrob Agents Chemother44775777CrossRefPubMedGoogle Scholar
  19. 19.
    Teuber, M, Meile, L, Schwarz, F 1999Acquired antibiotic resistance in lactic acid bacteria from foodAntonie van Leeuwenhoek76115137CrossRefPubMedGoogle Scholar
  20. 20.
    Yazid, AM, Ali, AM, Shuhaimi, M, Kalaivaani, V, Rokiah, MY, Reezal, A 2000Antimicrobial susceptibility of bifidobacteriaLett Appl Microbiol315762CrossRefPubMedGoogle Scholar
  21. 21.
    Young, JPW, Downer, HL, Eardly, BD 1991Phylogeny of the prototrophic Rhizobium strain BTAil by polymerase chain reaction-based sequencing of a 16S rRNA segmentJ Bacteriol17322712277PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Susana Delgado
    • 1
  • Ana Belén Flórez
    • 1
  • Baltasar Mayo
    • 1
  1. 1.Instituto de Productos Lácteos de Asturias (CSIC)AsturiasSpain

Personalised recommendations