Current Microbiology

, Volume 49, Issue 6, pp 415–422 | Cite as

Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil

  • Patcharaporn Wongsa
  • Makiko Tanaka
  • Akio Ueno
  • Mohammad Hasanuzzaman
  • Isao Yumoto
  • Hidetoshi Okuyama


Bacteria possessing high capacity to degrade gasoline, kerosene, diesel oil, and lubricating oil were screened from several areas of Hokkaido, Japan. Among isolates, two strains, WatG and HokM, which were identified as new strains of Pseudomonas aeruginosa and Serratia marcescens species, respectively, showed relatively high capacity and wide spectrum to degrade the hydrocarbons in gasoline, kerosene, diesel, and lubricating oil. About 90–95% of excess amount of total diesel oil and kerosene added to mineral salts media as a sole carbon source could be degraded by WatG within 2 and 3 weeks, respectively. The same amount of lubricating oil was 60% degraded within 2 weeks. Strain HokM was more capable than WatG in degrading aromatic compounds in gasoline. This strain could also degrade kerosene, diesel, and lubricating oil with a capacity of 50–60%. Thus, these two isolates have potential to be useful for bioremediation of sites highly contaminated with petroleum hydrocarbons.


Kerosene Petroleum Product Petroleum Hydrocarbon Mineral Salt Medium Serratia Marcescens 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was partially supported by Northern Advancement Center for Science & Technology.

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Copyright information

© Springer Science+Business Media Inc. 2004

Authors and Affiliations

  • Patcharaporn Wongsa
    • 1
    • 2
  • Makiko Tanaka
    • 1
    • 3
  • Akio Ueno
    • 1
    • 2
  • Mohammad Hasanuzzaman
    • 1
    • 2
  • Isao Yumoto
    • 3
  • Hidetoshi Okuyama
    • 2
  1. 1.ROM Co. Ltd.Chuo-kuJapan
  2. 2.Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth ScienceHokkaido UniversityKita-kuJapan
  3. 3.Institute for Biological Resource and FunctionNational Institute of Advanced Industrial Science and TechnologyToyohira-kuJapan

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