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Current Microbiology

, Volume 49, Issue 4, pp 274–281 | Cite as

MAP Kinase and cAMP Signaling Pathways Modulate the pH-Induced Yeast-to-Mycelium Dimorphic Transition in the Corn Smut Fungus Ustilago maydis

  • Alfredo D. Martínez-Espinoza
  • José Ruiz-Herrera
  • Claudia G. León-Ramírez
  • Scott E. Gold
Article

Abstract

Acid pH induces the yeast-to-mycelium transition in haploid cells of Ustilago maydis. We tested two signal transduction pathways known to be involved in dimorphism for roles in acid-induced filamentation. In wild-type cells intracellular cAMP levels were reduced under acid growth. A mutant defective in the regulatory subunit of PKA, ubc1, failed to respond to acid induction on solid medium, but in liquid medium showed a mycelial phenotype at acid pH. Mutants in the pheromone-responsive MAP kinase pathway lost the capacity to grow as mycelium at acid pH, while a mutant in the pheromone response-transcriptional regulator, prf1, behaved as wild-type. Filamentation by both ubc1 and prf1 mutants was inhibited by addition of cAMP. A putative MAP kinase cascade adaptor protein gene, ubc2, complemented a previously identified myc mutant strain defective in pH-induced myceliation. These results indicate that pH-dependent dimorphism is regulated by two known signaling pathways but that an effector for cAMP signaling alternative to Ubc1 is present in U. maydis and that Prf1 is not the sole downstream target of MAP kinase signaling.

Keywords

Intracellular cAMP cAMP Signaling Haploid Cell Intracellular cAMP Level Smut Fungus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Alfredo D. Martínez-Espinoza
    • 1
  • José Ruiz-Herrera
    • 2
  • Claudia G. León-Ramírez
    • 2
  • Scott E. Gold
    • 1
  1. 1.Department of Plant Pathology, University of Georgia, Athens, GA 30602-7274United States
  2. 2.Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 629, 36500 Irapuato, Gto.Mexico

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