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Current Microbiology

, Volume 45, Issue 6, pp 0459–0461 | Cite as

Isolation and Characterization of the Genes Encoding Δ8-Sphingolipid Desaturase from Saccharomyces kluyveri and Kluyveromyces lactis

  • Naoya Takakuwa
  • Mikio Kinoshita
  • Yuji Oda
  • Masao Ohnishi

Abstract

Saccharomyces kluyveri IFO 1685 and Kluyveromyces lactis IFO 1090 synthesize cerebroside containing 9-methyl-trans-4, trans-8-sphingadienine as a sphingoid base. From the genome of the two strains, the regions encompassing Δ8-sphingolipid desaturase were amplified and sequenced. The nucleotide sequences of these regions revealed single open reading frames of 1707 bp for S. kluyveri and 1722 bp for K. lactis, encoding polypeptides of 568 and 573 amino acids with molecular weights of 66.5 and 67.1 kDa, respectively. Conversion of 4-hydroxysphinganine to 4-hydroxy-trans-8-sphingenine in the cells of Saccharomyces cerevisiae was observed by the expressed gene from K. lactis and not by that from S. kluyveri. These findings may be explained by the difference in substrate specificity for the sphingoid base moiety between Δ8-sphingolipid desaturases of S. kluyveri and K. lactis.

Keywords

Molecular Weight Nucleotide Nucleotide Sequence Polypeptide Saccharomyces Cerevisiae 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Naoya Takakuwa
    • 1
  • Mikio Kinoshita
    • 1
  • Yuji Oda
    • 2
  • Masao Ohnishi
    • 1
  1. 1.Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, JapanJP
  2. 2.Department of Upland Agriculture Research, National Agricultural Research Center for Hokkaido Region, Memuro, Kasai, Hokkaido 082-0071, JapanJP

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