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Current Microbiology

, Volume 46, Issue 1, pp 0033–0038 | Cite as

Cloning and Characterization of Two Novel Crystal Protein Genes from a Bacillus thuringiensis serovar dakota Strain

  • Ho San Kim
  • Hiroyuki Saitoh
  • Satoko Yamashita
  • Tetsuyuki Akao
  • Yu Shin Park
  • Minoru Maeda
  • Rie Tanaka
  • Eiichi Mizuki
  • Michio Ohba

Abstract

Two genes encoding the 32- and 40-kDa polypeptides of Bacillus thuringiensis strain 90-F-45-14 crystals were cloned, expressed in an acrystalliferous B. thuringiensis strain, and sequenced. The polypeptides had deduced molecular weights of 30,319 and 33,885, respectively. The amino acid sequence of the 32-kDa protein was 37.7% identical to the known sequence of a non-insecticidal parasporal protein in B. thuringiensis serovar thompsoni crystals. The sequence of the cloned 40-kDa protein was 37.0% and 22.3% identical to that of the existing Cry protein classes, Cry15Aa1 and Cry23Aa1, respectively. Thus, this protein constitutes a novel protein class, Cry33Aa1. The open reading frames of the two genes were located on the predominant plasmid of 17,629 bp (=11,752 MDa) in the same orientation, and they were separated by the sequence of 32 nucleotides. The two proteins are likely produced simultaneously from a single transcript to form spherical crystals.

Keywords

Molecular Weight Nucleotide Amino Acid Sequence Bacillus Polypeptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Ho San Kim
    • 1
  • Hiroyuki Saitoh
    • 1
  • Satoko Yamashita
    • 1
  • Tetsuyuki Akao
    • 1
  • Yu Shin Park
    • 1
  • Minoru Maeda
    • 3
  • Rie Tanaka
    • 3
  • Eiichi Mizuki
    • 1
  • Michio Ohba
    • 2
  1. 1.Biotechnology & Food Research Institute, Fukuoka Industrial Technology Center, Aikawa-machi 1465-5 Kurume, Fukuoka 839-0861, JapanJP
  2. 2.Graduate School of Agriculture, Kyushu University, Fukuoka 812-8581, JapanJP
  3. 3.Section of Microbiology, Kyushu Medical Co., Ltd., Kitakyushu, Fukuoka 803-0814, JapanJP

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