Advertisement

Current Microbiology

, Volume 46, Issue 1, pp 0006–0010 | Cite as

Ecophysiological Variabilities in Ectohydrolytic Enzyme Activities of Some Pseudoalteromonas Species, P. citrea, P. issachenkonii, and P. nigrifaciens

  • Elena P. Ivanova
  • I. Yu. Bakunina
  • Olga I. Nedashkovskaya
  • Nataliya M. Gorshkova
  • Yulia V. Alexeeva
  • Elena A. Zelepuga
  • T.N. Zvaygintseva
  • Dan V. Nicolau
  • V.V. Mikhailov

Abstract

The ecophysiological variabilities in the ectohydrolytic enzyme profiles of the three species of Pseudoalteromonas, P. citrea, P. issachenkonii, and P. nigrifaciens, have been investigated. Forty-one bacteria isolated from several invertebrates, macroalgae, sea grass, and the surrounding water exhibited different patterns of hydrolytic enzyme activities measured as the hydrolysis of either native biopolymers or fluorogenic substrates. The activities of the following enzymes were assayed: proteinase, tyrosinase, lipase, amylase, chitinase, agarase, fucoidan hydrolase, laminaranase, alginase, pustulanase, cellulase, β-glucosidase, α- and β-galactosidases, β-N-acetylglucosaminidase, β-glucosaminidase, β-xylosidase, and α-mannosidase. The occurrence and cell-specific activities of all enzymes varied over a broad range (from 0 to 44 μmol EU per hour) and depended not only on taxonomic affiliation of the strain, but also on the source/place of its isolation. This suggests ‘specialization’ of different species for different types of polymeric substrates as, for example, all strains of P. citrea and P. issachenkonii hydrolyzed alginate and laminaran, while strains of P. nigrifaciens were lacking the ability to hydrolyze most of the algal polysaccharides. The incidence of certain enzymes such as fucoidan hydrolases, alginate lyases, agarases, and α-galactosidases might be strain specific and reflect its particular ecological habitat.

Keywords

Lipase Cellulase Alginate Amylase Macroalgae 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Elena P. Ivanova
    • 1
  • I. Yu. Bakunina
    • 1
  • Olga I. Nedashkovskaya
    • 1
  • Nataliya M. Gorshkova
    • 1
  • Yulia V. Alexeeva
    • 1
  • Elena A. Zelepuga
    • 1
  • T.N. Zvaygintseva
    • 1
  • Dan V. Nicolau
    • 2
  • V.V. Mikhailov
    • 1
  1. 1.Pacific Institute of Bio-organic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, 690022, Vladivostok, RussiaRU
  2. 2.Industrial Research Institute Swinburne, Swinburne University of Technology, PO Box 218, Hawthorn, Vic 3122, AustraliaAU

Personalised recommendations