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Current Microbiology

, Volume 44, Issue 1, pp 31–37 | Cite as

4-Hydroxybenzoate Uptake in Klebsiella planticola Strain DSZ1 Is Driven by ΔpH

  • J.L. Allende
  • A. Gibello
  • A. Fortún
  • M. Sánchez
  • M. Martín

Abstract

Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 × 10−6 (cpm/OD) h−1, and a rate of 4-HBA utilisation of 0.75 mmol h−1. Aerobically the 4HBA transport system is driven by gradient of protons (ΔpH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 μM with a Vmax value of 25 nmol/min.mg at pH 7.

Keywords

Nitrate Respiration Electron Acceptor Anaerobic Condition Aromatic Compound 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • J.L. Allende
    • 1
  • A. Gibello
    • 2
  • A. Fortún
    • 1
  • M. Sánchez
    • 3
  • M. Martín
    • 3
  1. 1.Dpt. Fisica Aplicada, Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, SpainES
  2. 2.Dpt. Patologia Animal I (Microbiología), Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, SpainES
  3. 3.Dpt. Bioquímica y Biología Molecular, Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, SpainES

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