4-Hydroxybenzoate Uptake in Klebsiella planticola Strain DSZ1 Is Driven by ΔpH
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Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 × 10−6 (cpm/OD) h−1, and a rate of 4-HBA utilisation of 0.75 mmol h−1. Aerobically the 4HBA transport system is driven by gradient of protons (ΔpH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 μM with a Vmax value of 25 nmol/min.mg at pH 7.
KeywordsNitrate Respiration Electron Acceptor Anaerobic Condition Aromatic Compound
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