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Seminars in Immunopathology

, Volume 40, Issue 6, pp 605–615 | Cite as

Picket-fences in the plasma membrane: functions in immune cells and phagocytosis

  • Sivakami M. Mylvaganam
  • Sergio Grinstein
  • Spencer A. FreemanEmail author
Review

Abstract

Recent studies of molecular mobility in the plasma membrane have revealed that diffusion is restricted by cytoskeletal networks or fences. Transmembrane protein “pickets” that reversibly associate with the membrane-associated skeleton and with the pericellular coat impede the movement of unattached bystander molecules. While membrane picket-fences were originally described as barriers to free diffusion in more passive cell types such as fibroblasts, they have particularly important functions in the more dynamic immune cells. In phagocytes, such fences curtail spontaneous activation and their disassembly facilitates stimulation by target particles, fostering receptor clustering and the exclusion of phosphatases from the phagocytic cup. In this review, we describe the nature of the cellular cytoskeleton and of the exoskeleton created by the pericellular coat, their association with transmembrane pickets, and the modulation of molecular mobility during phagocytosis.

Keywords

Macrophage Cytoskeleton F-actin Spectrin Glycocalyx Single-particle tracking Diffusion CD44 CD45 

Notes

Funding information

S.A.F. is supported by a Banting fellowship of the Canadian Institutes of Health Research (CIHR). S.G. is supported by grant FDN-143202 from CIHR.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Program in Cell Biology, Peter Gilgan Centre for Research and LearningHospital for Sick ChildrenTorontoCanada
  2. 2.Department of BiochemistryUniversity of TorontoTorontoCanada
  3. 3.Keenan Research Centre of the Li Ka Shing Knowledge InstituteSt. Michael’s HospitalTorontoCanada

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