Cancer Chemotherapy and Pharmacology

, Volume 84, Issue 6, pp 1315–1321 | Cite as

Combined thermo-chemotherapy of cancer using 1 MHz ultrasound waves and a cisplatin-loaded sonosensitizing nanoplatform: an in vivo study

  • Rasoul Irajirad
  • Amirhossein Ahmadi
  • Bahareh Khalili Najafabad
  • Ziaeddin Abed
  • Roghayeh Sheervalilou
  • Samideh Khoei
  • M. Bagher Shiran
  • Habib GhaznaviEmail author
  • Ali Shakeri-ZadehEmail author
Original Article



The aim of the present study was to develop a new strategy for combined thermo-chemotherapy of cancer. For this purpose, we used ultrasound waves [1 MHz; 1 W/cm2; 10 min] in combination with a sonosensitizing nanoplatform, named ACA, made of alginate co-loaded with cisplatin and gold nanoparticles (AuNPs).


Various combinatorial treatment regimens consisting of ultrasound, AuNPs, cisplatin, and ACA nanoplatform were studied in vivo. The CT26 colon adenocarcinoma cell line was used for tumor induction in BALB/c mice. During the ultrasound exposure, we monitored the temperature variations in each treatment group using infrared thermal imaging. Furthermore, tumor metabolism was assessed by [18F]FDG (2-deoxy-2-[18F]fluoro-d-glucose)-positron emission tomography (PET) imaging.


The combination of ultrasound with nanoplatform showed an improved therapeutic efficacy than free cisplatin or ultrasound alone. It was revealed that the examined thermo-chemotherapy protocol has the potential to intensively decrease the metabolic activity of CT26 tumors.


The data obtained in this study confirmed a potent anti-tumor efficacy caused by the ACA nanoplatform and ultrasound combination. It may provide a beneficial cancer therapy strategy in which the thermal and mechanical effects of ultrasound can intensify the therapeutic ratio of conventional chemotherapy methods.


Cancer Chemotherapy Nanotechnology Hyperthermia Combination therapy 



All financial supports received from Iran University of Medical Sciences (Grant no. 29865) and Zahedan University of Medical Sciences (Grant no. 7970) are acknowledged.

Compliance with Ethical Standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in this study were in accordance with the ethical standards of Iran University of Medical Sciences (IUMS ethics committee, Permit number: IR.IUMS.REC 1395.95-03-30-27720).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Medical Physics Department, School of MedicineIran University of Medical Sciences (IUMS), Hemmat Exp.TehranIran
  2. 2.Faculty of Pharmacy, Pharmaceutical Sciences Research CenterMazandaran University of Medical SciencesSariIran
  3. 3.Cellular and Molecular Research CenterZahedan University of Medical Sciences (ZaUMS)ZahedanIran

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