Treatment with selenium-enriched Saccharomyces cerevisiae UFMG A-905 partially ameliorates mucositis induced by 5-fluorouracil in mice

  • Bárbara A. A. Porto
  • Cinthia F. Monteiro
  • Éricka L. S. Souza
  • Paola C. L. Leocádio
  • Jacqueline I. Alvarez-Leite
  • Simone V. Generoso
  • Valbert N. Cardoso
  • Camila M. Almeida-Leite
  • Daniel A. Santos
  • Julliana R. A. Santos
  • Jacques R. Nicoli
  • Enrica Pessione
  • Flaviano S. MartinsEmail author
Original Article



Gastrointestinal mucositis is a major problem associated with cancer therapy. To minimize these deleterious effects, simultaneous administration of antioxidant components, such as selenium, can be considered. There is a growing interest in the use of yeasts because they are able to convert inorganic selenium into selenomethionine. In the present study, oral administration of Saccharomyces cerevisiae UFMG A-905 enriched with selenium was evaluated as an alternative in minimizing the side effects of 5FU-induced mucositis in mice.


Mice body weight, food consumption, faeces consistency and the presence of blood in faeces were assessed daily during experimental mucositis induced by 5-fluorouracil (5FU). Blood was used for intestinal permeability determination, and small intestine for oxidative stress, immunological and histopathological examination.


The increased intestinal permeability observed with mucositis induction was partially reverted by S. cerevisiae and selenium-enriched yeast. Both treatments were able to reduce myeloperoxidase activity, but only selenium-enriched yeast reduced eosinophil peroxidase activity. CXCL1/KC levels, histopathological tissue damage and oxidative stress (lipid peroxidation and nitrite production) in the small intestine were reduced by both treatments; however, this reduction was always higher when treatment with selenium-enriched yeast was evaluated.


Results of the present study showed that the oral administration of S. cerevisiae UFMG A-905 protected mice against mucositis induced by 5-FU, and that this effect was potentiated when the yeast was enriched with selenium.


Probiotics Saccharomyces cerevisiae UFMG A-905 Selenium Selenium-enriched yeast Mucositis 5-Fluorouracil 



This work was supported by grants from the Brazilian National Council for Scientific and Technological Development (CNPq), the Ministry of Science and Technology (MICT) and the Foundation for Research Support of the State of Minas Gerais (FAPEMIG—APQ-00593-14), Brazil. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. BAAP was the recipient of a Ph.D.’s fellowship from CNPq. Professors JIAL, VNC, DAS, JRN and FSM are CNPq fellowship holders.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Animal experiments were carried out according to the standards set forth by the Brazilian National Council for Control of Animal Experimentation (CONCEA). This study was approved under Protocol no. 186/2012 by the Ethics Committee on the Use of Animals (CEUA/UFMG).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Bárbara A. A. Porto
    • 1
  • Cinthia F. Monteiro
    • 1
  • Éricka L. S. Souza
    • 1
  • Paola C. L. Leocádio
    • 2
  • Jacqueline I. Alvarez-Leite
    • 2
  • Simone V. Generoso
    • 3
  • Valbert N. Cardoso
    • 4
  • Camila M. Almeida-Leite
    • 5
  • Daniel A. Santos
    • 1
  • Julliana R. A. Santos
    • 6
  • Jacques R. Nicoli
    • 1
  • Enrica Pessione
    • 7
  • Flaviano S. Martins
    • 1
    Email author
  1. 1.Department of Microbiology, Institute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil
  2. 2.Department of Biochemistry and Immunology, Institute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil
  3. 3.Department of Basic Nursing, School of NursingFederal University of Minas GeraisBelo HorizonteBrazil
  4. 4.Department of Clinical and Toxicological Analysis, Faculty of PharmacyFederal University of Minas GeraisBelo HorizonteBrazil
  5. 5.Department of Morphology, Institute of Biological SciencesFederal University of Minas GeraisBelo HorizonteBrazil
  6. 6.Laboratory of Environmental MicrobiologyCEUMA University (UNICEUMA)São LuísBrazil
  7. 7.Life Science and Systems Biology DepartmentUniversity of TorinoTorinoItaly

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