Efficacy of CR3294, a new benzamidine derivative, in the prevention of 5-fluorouracil-induced gastrointestinal mucositis and diarrhea in mice
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Gastrointestinal mucositis, commonly associated with diarrhea, is a dose-limiting toxicity of chemotherapy. The new benzamidine derivative CR3294 reduces tissue damage in animal models of intestinal inflammation. Thus, we tested whether CR3294 had the potential to prevent chemotherapy-induced mucositis.
In tests on isolated cells, reactive oxygen species (ROS) formation and cytokine release were measured by chemiluminescence and immunoassays, respectively. In studies in vivo, BDF1 mice were given oral CR3294 (2.5–20 mg/kg) for 3 days before receiving 5-fluorouracil. Intestinal crypt survival, cell apoptosis and proliferation, and diarrhea score were assessed. Additionally, nude mice bearing tumor xenografts were treated with CR3294 and/or 5-fluorouracil, and tumor growth was monitored.
CR3294 significantly inhibited cytokine release from stimulated leukocytes at concentrations similar to the IC50 (2.9 ± 0.2 μM) for ROS production by these cells. Consistent with these molecular findings, CR3294 dose-dependently protected the intestinal mucosa against 5-fluorouracil-induced toxicity in a mouse model of mucositis. The number of surviving crypts per cross-section in mice receiving 20 mg/kg CR3294 was 2.8-fold that in vehicle-treated animals (18.1 ± 1.9 vs. 6.5 ± 0.9, P < 0.001). Moreover, CR3294 decreased the cumulative diarrhea score by 50%, reduced by nearly 70% the incidence of severe episodes, and increased by 3-fold the number of mice without diarrhea. CR3294 neither affected the growth of tumor xenografts nor protected tumors from the cytotoxic activity of 5-fluorouracil.
This study demonstrates that CR3294 acts on key molecular targets to reduce the signs of mucositis and the occurrence of diarrhea in mice exposed to the chemotherapy drug 5-fluorouracil.
KeywordsCR3294 Mucositis Chemotherapy Diarrhea Cancer
Chemotherapy and radiation therapy are useful for treating many forms of cancer. However, they also affect rapidly dividing cells in healthy tissues, in particular the epithelial cells of the alimentary tract. This is the reason why oral and gastrointestinal (GI) mucositis commonly arises as an adverse effect of anticancer therapy and often causes dose delays or reductions, which can hamper treatment success [1, 2, 3].
GI mucositis is associated with many chemotherapy protocols and with radiotherapy directed to any part of the GI tract. These treatments kill progenitor cells in the crypts of Lieberkühn, at the base of the villi, with consequent breakdown of the mucosal barrier . Diarrhea is one of the most common manifestations of GI mucositis. The incidence and severity of diarrhea vary greatly, depending on the therapeutic regimen chosen. Chemotherapy-induced diarrhea (CID) occurs in as many as 80% of patients with cancer treated with 5-fluorouracil (5-FU) and irinotecan. Of even greater concern, increased death rates from GI toxicity have been reported in patients receiving irinotecan plus bolus 5-FU/leucovorin [3, 4, 5, 6, 7]. As the impact of CID rises with the advent of more aggressive anticancer therapies, new therapeutic options for preventing GI mucositis and diarrhea are awaited [5, 6, 8, 9].
CR3294 belongs to a novel series of anti-inflammatory agents that inhibit the expression of inducible nitric oxide synthase (iNOS). Notably, this compound protects the epithelium from injury in validated animal models of GI inflammation [10, 11]. Mucositis is now regarded as a complex phenomenon in which many cellular and tissue components of the mucosa play a part. In essence, reactive oxygen species (ROS) activate downstream signaling pathways, transcription factors mediate the expression of target genes such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), and these cytokines fuel a vicious loop that harms mucosal integrity [12, 13, 14, 15]. On the basis of the evidence available and because of the unmet medical need, CR3294 was tested as a preventive agent against mucositis.
The aims of this study were to provide in vitro data that support a role for CR3294 in reducing the molecular triggers for mucositis and to assess, in a relevant animal model, whether CR3294 has the potential to prevent GI mucositis and diarrhea.
Materials and methods
CR3294, N-[4-[[(pentylamino)thioxomethyl]amino]phenyl]-ethanimidamide, monohydrochloride, was synthesized by the Medicinal Chemistry Department of Rottapharm S.p.A. (Monza, Italy). Phorbol-12-myristate-13-acetate (PMA), luminol, HISTOPAQUE-1077, and lipopolysaccharide (LPS) were from Sigma (Milan, Italy); dextran T500 was from Amersham Pharmacia Biotech (Uppsala, Sweden); and multiplex human cytokine kits were from Bio-Rad (Milan, Italy). All general purpose reagents used in animal studies were from Sigma–Aldrich (Poole, UK). Animals were purchased from Harlan Laboratories UK Ltd., and tumor cell lines were from the American Type Culture Collection (ATCC). We purchased 5-FU from Roche (Welwyn Garden City, UK). Rat anti-5-bromo-2′-deoxy-uridine (BrdU) clone U1/75 (ICR1) antibody was from AbD SeroTec MorphoSys UK Ltd., with labeling detected using the ABC Elite system from Vector Laboratories UK Ltd.
In vitro studies
ROS production by human polymorphonuclear leukocytes (neutrophils)
Venous blood was collected from healthy human donors in sodium citrate 0.38% and centrifuged at 275g for 15 min. The resulting buffy coat was diluted 3 times (3–9 mL) with phosphate-buffered saline (PBS) and mixed with 2 mL of 6% dextran T500 in 0.9% saline. This mixture was left for 45 min at room temperature, then the supernatant was collected and centrifuged at 275g for 15 min. The pellet (leukocytes) was subjected to hypotonic lysis of contaminating erythrocytes by addition of distilled water. The osmolarity was restored with 0.8% NaCl solution. Leukocytes were then centrifuged at 275g for 15 min and washed again in PBS. Polymorphonuclear leukocytes (PMNL) were isolated by gradient centrifugation (400g for 30 min) on HISTOPAQUE-1077. Purified PMNL were suspended in PBS plus 0.87 mM CaCl2 and 1 mM MgCl2, counted, and diluted to 2.5 × 106 cells/mL. For chemiluminescence assays, the PMNL suspension was premixed with luminol (5 μM final). Aliquots of cell suspension (200 μL) were incubated with CR3294 (1, 3, or 10 μM) or its vehicle (distilled water) for 10 min at 37°C. Next, PMNL were activated with 0.1 μM PMA, and light emission was monitored at 3-min intervals for 24 min in a HTS7000 plus microplate reader. Results are expressed as relative luminescence units (RLU).
Cytokine release from peripheral blood mononuclear cells
Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation on HISTOPAQUE-1077, according to the manufacturer’s instructions. Purified cells were resuspended in RPMI-1640 culture medium containing 1% fetal bovine serum and adjusted to 2 × 106 cells/mL. Aliquots of cell suspension (200 μL) were added to duplicate wells of a flat-bottom 96-well plate containing CR3294 or vehicle (distilled water) and incubated for 1 h at 37°C in a humidified atmosphere of 5% CO2. Cytokine synthesis was induced by a 4-h stimulation with 1 μg/mL LPS (from E. coli, serotype 0127B7). To rule out cytotoxic effects, parallel plates were tested for viability by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. At the end of the incubation period, supernatants were collected and assayed for IL-1β, TNF-α, IL-6, and IL-8 by Bio-Plex technology (BioRad, Milan, Italy) according to the recommended procedures. Data were analyzed using the Bio-Plex Manager™ software from Bio-Rad (version 4.0), and results are given as IC50 values (μM).
In vivo studies
Ethics and animal care
All studies were conducted in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986, at standards equivalent to the UKCCCR guidelines for the welfare of animals in experimental neoplasia . Animals were held in individually ventilated cages in a specific pathogen-free barrier unit. On arrival from the supplier, mice were housed for 2 weeks to enable acclimatization and stabilize the circadian rhythm. Mice were identified by numbered cages and by ear punches. There was a constant room temperature of 21 ± 2°C and a mean relative humidity of 55 ± 10%. The day–night cycle was constant, with light and dark phases of 12 h each. Both feed and water (from drinking bottles) were available ad libitum. Animal health was monitored daily and cages were cleaned at regular intervals.
Analysis of intestinal crypt survival
Male BDF1 mice (10–12 weeks of age, n = 8 per group) were used in this experiment. CR3294 was stored at room temperature in the dark: solutions were prepared fresh each day in deionized water, and concentrations were adjusted to give the appropriate dose (2.5, 5, 10, or 20 mg/kg) in 100 μL. Intestinal damage was induced by either 5-FU (2 × 450 mg/kg intraperitoneally, 6 h apart) or irradiation (13 Gy) on day 0 of the study, after 3 days of once-daily oral administration of CR3294 or its vehicle. Irradiation was administered by a Pantak HF320 X-ray unit (Agfa NDT Ltd., Reading, UK). The machine was operated at 300 kV, 10 mA. The X-ray tube was fitted with additional filtration to give a radiation quality of 2.3-mm Cu half-value layer (HVL). Mice were restrained in a jig positioned at a distance of 700 mm from the focus of the X-ray tube, and irradiation was delivered at a dose rate of 70 cGy/min.
The effects of CR3294 were also expressed as Protection Index, i.e., the ratio of surviving crypts in treatment group to surviving crypts in vehicle control group.
Cell proliferation and apoptosis
Male BDF1 mice (10–12 weeks of age, n = 6 per group) were randomized into 7 groups and divided into 2 experiments. In the first one, we tested the effect of CR3294 (20 mg/kg) on crypt cell proliferation 24 h after 3 daily doses (i.e., the time at which 5-FU is administered). Animals were dosed with 10 mg of BrdU (0.5 mL intraperitoneally) 40 min before killing. In the second experiment, we examined the levels of apoptosis 4.5 h after exposure to single or repeated (2 times, 6 h apart) cytotoxic doses of 5-FU (450 mg/kg). Mice were killed by cervical dislocation in both studies. The small intestine was removed, fixed in Carnoy’s fixative, paraffin embedded, and sectioned to generate 2 non-serial sections per slide. Carnoy’s fixed sections were either labeled to reveal BrdU incorporation or H&E stained to measure the incidence of apoptosis. Crypts were analyzed on a cell positional basis to identify drug-induced changes. In all cases, 50 half-crypts per mouse were scored—300 scores per group, from which the frequencies were generated . Quantification of BrdU labeling and apoptosis was performed by the purpose-built WinCrypts software program, which analyzes the statistical significance of any distributions using an extension of the median test. At each cell position, the common median for all the animals was calculated, and then the individual values for each animal were classified as being below or above the median .
Male BDF1 mice (10–12 weeks of age, n = 15 per group) were randomized into 2 groups. Dosage regimens for 5-FU and CR3294 (20 mg/kg) were consistent with those used in the experiments described earlier. Mice were weighed once-daily after the beginning of treatment. Similarly, animal well-being was checked daily, and the incidence and severity of diarrhea were scored. Checks increased to twice a day during days 4–11. Scores were recorded as 0, 1, 2, or 3 where 0 is normal stool consistency, 1 is loose stools, 2 is overt diarrhea, and 3 is liquid feces with severe perianal/tail soilage (extreme diarrhea). Surviving animals were to be killed 20 days after the cytotoxic insult.
Tumor proliferation models
Two experiments were carried out to rule out adverse effects of CR3294 on tumor growth or 5-FU activity. In the first study, 16 male and 16 female BALB/c nude mice were xenografted with human colorectal (HT29) and breast (MDA-MB-231) cancer cells, respectively. Tumors were measured with calipers 3 times per week and were allowed to grow until the mean tumor volume of the group reached 100–150 mm3. Animals were treated with 20 mg/kg CR3294 or vehicle (sterile water) daily for 14 days. In the second study, 32 male BALB/c nude mice were xenografted with the colorectal HT29 cell line and randomized into 4 treatment groups. Groups 2 and 4 were treated with oral CR3294 on days 1–3 and 8–10 (incorporating a 5-day interval). The cytotoxic treatment (5-FU, 25 mg/kg intraperitoneally) was administered on days 1–5 in group 4 as well as in cytotoxic control group 3. The dual vehicle control group (group 1) was administered with the test agent vehicle (water) on days 1–3 and 8–10 and with the 5-FU vehicle (saline) on days 1–5. All the mice in both studies were weighed 3 times per week. Well-being was checked daily: any animal with more than 15% weight loss was considered unwell and killed if the weight loss was greater than 20%. If an animal became unwell, any treatment of that animal was suspended. If there was no recovery, the mouse was killed. Tumor sizes were measured for up to 28 days after the first dose of CR3294 or vehicle.
For the evaluation of statistical differences between experimental groups, one- or two-way analysis of variance (ANOVA) followed by Holm–Sidak test for multiple comparisons were used. Data without homogeneity of variance were analyzed with Mann–Whitney rank sum test or Kruskal–Wallis one-way ANOVA, and multiple post hoc comparisons were performed using Dunn’s method. In studies of proliferation and apoptosis, the common median for all the animals (at each cell position in the crypt) was calculated. Next, the individual values for each animal were classified as being below or above the median, and the chi-squared test was applied on the tabulated frequencies. All analyses were done with the SigmaStat version 3.5 software, and a significance level of 0.05 was used throughout.
In vitro studies
First, we tested the hypothesis that CR3294 has the ability to inhibit key molecular events in the onset of mucositis. In these in vitro studies, we used human PMNL and PBMC because leukocytes are a rich source of both ROS and cytokines.
To assess the impact of CR3294 on cytokine release, human PBMC were stimulated with 1 μg/mL LPS for 4 h. Under these conditions, CR3294 concentration-dependently inhibited the release of TNF-α, IL-6, and IL-8. The IC50 values ranged between 6 and 9 μM. Moreover, CR3294 potently inhibited the release IL-1β, with an IC50 of about 100 nM. Similar results were obtained in two independent experiments, each done in duplicate, and significant inhibitions were generally seen at concentrations of 3 μM or higher.
In vivo studies
Effects of CR3294 on the mucosal epithelium
Effect of pretreatment with CR3294 on 5-FU-induced intestinal crypt death
Surviving crypts per circumferenceb, mean ± SE
Protection Index, fold change compared to vehicle-treated controls
6.5 ± 0.9
CR3294 2.5 mg/kg
9.9 ± 1.0
CR3294 5 mg/kg
11.7 ± 2.5
CR3294 10 mg/kg
11.8 ± 1.2
CR3294 20 mg/kg
18.1 ± 1.9*
Effect of CR3294 on 5-FU-induced diarrhea
Animals died between days 8 and 13 after 5-FU challenge. Thus, CR3294 did not improve survival, although the onset of death was slightly delayed in the group receiving the active treatment (4 deaths during days 8 and 9 vs. 7 deaths during the same period in the vehicle-treated group).
Tumor proliferation models
Severe alimentary mucositis has a major impact on patients undergoing chemotherapy or radiation therapy. There is clearly an unmet medical need for drugs that reduce the rate and severity of GI mucositis and diarrhea . Indeed, clinical practice guidelines call for sustained efforts to develop better therapeutic strategies, targeted to the mechanisms of mucosal injury . The present experimental study demonstrates that CR3294 improves the signs and symptoms of GI mucositis by blocking key molecular events in the pathogenesis of this condition.
CR3294 is an anti-inflammatory agent that inhibits the expression of iNOS and cyclooxygenase 2 (COX-2) . Recent findings suggest a role for iNOS and COX-2 in the onset of mucositis [12, 14, 23]. However, it is widely accepted that ROS initiate the cascade of events—including increased levels of inflammatory cytokines—that leads to mucosal damage . In this study, CR3294 inhibited the release of IL-1β, IL-6, IL-8, and TNF-α from stimulated leukocytes at concentrations similar to those required to inhibit ROS production by these cells. The link between oxidative stress and mucositis is confirmed by other studies in which ROS scavengers were tested as protective agents against radiation-induced damage [24, 25]. Palifermin, the only drug approved for the treatment of oral mucositis, is a human keratinocyte growth factor (KGF) produced by recombinant DNA technology. In addition to stimulating epithelial cell proliferation, KGF activates the transcription factor NF-E2-related factor 2 (Nrf2), which in turn increases the expression of ROS-scavenging enzymes . Because the use of anti-inflammatory agents is now regarded as a promising approach to reduce the severity of mucositis , there is a strong rationale for developing new drugs with combined ROS-scavenging and anti-inflammatory properties. This is particularly relevant to GI mucositis, for which no therapy is currently approved although a variety of agents have been assessed in preclinical and clinical studies. Recently, alternative medicine approach gained attention. Probiotics have been shown to reduce intestinal inflammation and diarrhea in experimental models of GI mucositis in rats [28, 29, 30]. Herbal extracts, due to their anti-inflammatory and anti-oxidative properties and in keeping with CR3294 activity, have been shown to decrease mucosal damage in preclinical and clinical studies [31, 32].
The main goal of this study was to examine whether CR3294 has the potential to prevent GI mucositis and diarrhea. In a first set of in vivo experiments, we found that CR3294 significantly protected crypt cells against damage induced by 5-FU. Conversely, no definite effects were seen in an experimental model of radiation-induced mucositis. Different factors could account for the lack of conclusive results in this experimental setting. First, the adopted protocol may be non-optimal. Further studies with different radiation schemes have been planned to test the above hypothesis. Additionally, because X-rays and 5-FU target different cells in the intestinal crypts , it is conceivable that CR3294 protects a specific cell type in the crypt hierarchy. Unlike KGF, CR3294 did not stimulate epithelial cell proliferation, but similarly to KGF, the effectiveness of CR3294 was greatest when it was administered in a preventive mode . This finding suggests that other mechanisms, besides ROS scavenging, contribute to the cytoprotective activity of this agent. One such mechanism may involve decreased sensitivity to 5-FU-induced apoptosis , but our experiments did not show significant differences in this regard. Alternatively, given that CR3294 can induce the formation of autophagosomes , it is possible that CR3294 increased crypt survival by activating the autophagic pathway. Autophagy may indeed serve as a rescue mechanism whereby cells extend their lifespan under critical conditions . Although analyzing autophagy-related processes was beyond the scope of our present work, studies are under way to test the above hypothesis.
Here, we were interested in assessing whether mucosal protection could translate into a lower incidence of diarrhea, because CID is associated with dose reductions, dose delays, and excess hospitalizations and deaths. In our proof-of-concept study, pretreatment with CR3294 halved the cumulative diarrhea score in mice exposed to 5-FU. CR3294 was particularly effective against moderate-to-severe CID: animals treated with the 20 mg/kg dose were 70% less likely to experience grade 2–3 diarrhea than vehicle-treated mice. Severe diarrhea is common in patients receiving fluoropyrimidine-based chemotherapy [4, 6, 37]. In the absence of effective drugs to avoid this adverse event, treatment has superseded prevention in the clinical setting, and high-dose loperamide is used for managing mild-to-moderate diarrhea. Unfortunately, loperamide is far less effective when it comes to severe episodes, those that may be life threatening [7, 37, 38].
CR3294 did not improve animal survival, even though it slightly delayed the onset of death after exposure to 5-FU. However, our proof-of-concept study was designed to detect an effect of the compound on diarrhea rate and severity rather than on death from GI syndrome. Damage to the GI tract, if sufficiently serious, would cause death within 6–7 days, whereas none of the mice in our study died before day 8 after 5-FU injection. So, mortality was presumably associated with damage to the hematopoietic system ; and clearly, CR3294 did not limit chemical-induced toxicity to the bone marrow.
Likewise, CR3294 neither altered the antitumor activity of 5-FU nor affected the growth rate of tumor xenografts in both female and male mice. Candidate drugs for the prevention or treatment of mucositis must not protect tumors. For instance, other authors have demonstrated that palifermin does not stimulate the growth of tumors, even those expressing the KGF receptor [29, 40]. Regardless that CR3294 is unlikely to promote cell proliferation via KGF-dependent mechanisms, we ruled out unexpected yet possible untoward effects. A recent study has even shown that CR3294 reduces tumor growth in vivo . The variable results between that study and the present one might be due either to the different models used or to the different duration of treatment (8 vs. 2 weeks).
In summary, our experimental study demonstrates that CR3294 acts on critical mechanisms of mucosal injury to reduce the signs of GI mucositis as well as the incidence and severity of 5-FU-induced diarrhea. These positive results warrant further investigation in humans because no drugs are currently approved for the prevention of CID. Phase I clinical trials are now under way to evaluate the safety profile of CR3294.
The authors thank Mr. Mario Montagna for technical support and Mrs. Laura Radaelli for secretary assistance. This study was funded by the Rottapharm Group. However, the Rottapharm Group as a corporate entity had no role in the conduct of the study; in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. This work was part of a project co-financed in conjunction with the Italian Ministry of Health (Attività di ricerca finalizzata D. Lgs. 502/92).
Conflict of interest statement
All authors except Catherine Booth are employees of Rottapharm S.p.A. Catherine Booth is an employee of EpiStem Ltd., a research organization under contract with the Rottapharm Group.
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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