Mebendazole inhibits growth of human adrenocortical carcinoma cell lines implanted in nude mice
Adrenocortical carcinoma is a rare tumor of the adrenal gland which requires new therapeutic approaches as its early diagnosis is difficult and prognosis poor despite therapies used. Recently, mebendazole has been proved to be effective against different cancers. The aim of our study was to evaluate whether mebendazole may result therapeutically useful in the treatment of human adrenocortical carcinoma. We analyzed the effect of mebendazole on human adrenocortical carcinoma cells in vitro and after implantation in nude mice. In order to clarify mechanisms of mebendazole action, metastases formation, apoptosis and angiogenesis were also investigated. Mebendazole significantly inhibited cancer cells growth, both in vitro and in vivo, the effects being due to the induction of apoptosis. Moreover, mebendazole inhibited invasion and migration of cancer cells in vitro, and metastases formation in vivo. Overall, these data suggest that treatment with mebendazole, also in combination with standard therapies, could provide a new protocol for the inhibition of adrenocortical carcinoma growth.
KeywordsAdrenocortical carcinoma Mebendazole Nude mice Chemotherapy
- 2.Berruti A, Terzolo M, Sperone P, Pia A, Casa SD, Gross DJ, Carnaghi C, Casali P, Porpiglia F, Mantero F, Reimondo G, Angeli A, Dogliotti L (2005) Etoposide, doxorubicin and cisplatin plus mitotane in the treatment of advanced adrenocortical carcinoma: a large prospective phase II trial. Endocr Relat Cancer 12:657–666PubMedCrossRefGoogle Scholar
- 8.Gicquel C, Raffin-Sanson M, Gaston V, Bertagna X, Plouin P, Schlumberger M, Louvel A, Luton J, Le Bouc Y (1997) Structural and functional abnormalities at 11 p 15 are associated with the malignant phenotype in sporadic adrenocortical tumors. Study on a series of 82 tumors. J Clin Endocrinol Metab 82:2559–2565PubMedCrossRefGoogle Scholar
- 14.Norton JA (2005) Adrenal tumors. In: DeVita VT Jr, Hellman S, Rosenberg SA (eds) Cancer: principles and practice of oncology, 7th edn. Lippincott Williams & Wilkins, Philadelphia, pp 1528–1539Google Scholar
- 20.Zhiheng H, Bateman A (1999) Progranulin gene expression regulates epithelial cell growth and promotes tumor growth in vivo. Cancer Res 59:3222–3229Google Scholar