Cancer Chemotherapy and Pharmacology

, Volume 61, Issue 3, pp 459–469 | Cite as

Oligo-microarray analysis reveals the role of cyclophilin A in drug resistance

  • Shuai Chen
  • Mingjun Zhang
  • Honghui Ma
  • Hexige Saiyin
  • Suqin Shen
  • Jiajie Xi
  • Bo Wan
  • Long Yu
Original Article
First Online:
Received:
Accepted:

Abstract

Cyclophilin A (CYPA) belongs to peptidyl prolyl isomerases (PPIases), which catalyze the cis/trans isomerization of prolyl peptide bonds in cellular communication. CYPA has been implicated in several pathological processes, including cancer, inflammatory diseases, and HIV-1 infection. Up-regulation of CYPA has been found to be a common phenomenon in several tumor types, including in hepatocellular carcinoma (HCC). However, the role of CYPA in tumor cells remains unknown. We generated a stable SK-Hep1 cell line and studied the CYPA regulated genes at the transcriptome level. The microarray results reveal that CYPA can up-regulate the expression of many cytokine and drug resistance related genes. Furthermore, we showed that the elevated CYPA expression contributes to drug resistance. We postulate that the over-expression of CYPA in tumors may play a role in clinical resistance to chemotherapy.

Keywords

Oligo-microarray Peptidyl–prolyl isomerase (PPIase) Cyclophilin A (CYPA/PPIA) Hepatocellular carcinoma (HCC) Multi-drug resistant protien (MRP) 
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Notes

Acknowledgments

This work was supported by the Graduate Innovation Foundation of Fudan University (No. EYH1322021).

Supplementary material

280_2007_491_MOESM1_ESM.doc (107 kb)
The specificity of rabbit multiclonal antibody of CypA was evaluated by western blot. As shown in the figure, the specificity is high and no other band detected (DOC 107 kb)
280_2007_491_MOESM2_ESM.xls (573 kb)
ESM2 (XLS 573 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Shuai Chen
    • 1
  • Mingjun Zhang
    • 1
  • Honghui Ma
    • 1
  • Hexige Saiyin
    • 1
  • Suqin Shen
    • 1
  • Jiajie Xi
    • 1
  • Bo Wan
    • 1
  • Long Yu
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life SciencesFudan UniversityShanghaiPeople’s Republic of China

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