Cancer Chemotherapy and Pharmacology

, Volume 57, Issue 4, pp 458–464

A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of actinomycin-D and vincristine in children with cancer

  • Jeffrey M. Skolnik
  • Jeffrey S. Barrett
  • Heng Shi
  • Peter C. Adamson
Original Article

Abstract

Actinomycin-D (Act-D) and vincristine (VCR) are cytotoxic agents commonly used in the treatment of pediatric cancers. To date, there are few published methods on quantifying Act-D or VCR and no published methods on quantifying the two drugs together. We present a methodology for the simultaneous quantification of Act-D and VCR in human plasma using liquid chromatography-tandem mass spectrometry (LC/MS/MS) detection. Following solid phase extraction, plasma samples were separated and analyzed using electrospray ionization (ESI). The lower limit of quantitation (LLOQ) for both Act-D and VCR was 0.5 ng/ml. The analytical accuracy for detection of both Act-D and VCR was ≥ 90%. The analytical precision, as estimated by the coefficient of variation was ≤ 6% for Act-D and ≤ 11% for VCR. Given the prevalence of the use of the two drugs as combination therapy in a variety of pediatric oncological indications, the small sample volume requirements and the assay sensitivity, this methodology is expected to support several ongoing and future pediatric trials.

Keywords

Actinomycin-D Vincristine Method Validation Pediatric Pharmacokinetics 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jeffrey M. Skolnik
    • 1
    • 2
  • Jeffrey S. Barrett
    • 1
  • Heng Shi
    • 1
  • Peter C. Adamson
    • 1
    • 2
  1. 1.Division of Clinical Pharmacology and Therapeutics; Children’s Hospital of PhiladelphiaAbramson Research CenterPhiladelphiaUSA
  2. 2.Division of OncologyThe Children’s Hospital of PhiladelphiaPhiladelphiaUSA

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