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Cancer Chemotherapy and Pharmacology

, Volume 56, Issue 4, pp 436–446 | Cite as

Antiangiogenic and antitumoral properties of a polysaccharide isolated from the seaweed Sargassum stenophyllum

  • Paulo Fernando Dias
  • Jarbas Mota SiqueiraJr
  • Luiz Felipe Vendruscolo
  • Teresinha de Jesus Neiva
  • Antônio Ricardo Gagliardi
  • Marcelo Maraschin
  • Rosa Maria Ribeiro-do-ValleEmail author
Original Article

Abstract

The potential antiangiogenic and antitumoral properties of SargA, a polysaccharide extracted from the brown marine alga Sargassum stenophyllum, were studied in assays carried out in chick embryos and mice. Gelfoam plugs containing SargA (2–1500 μg/plug) implanted in vivo into fertilized 6-day-old chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). By day 8, the highest dose of SargA alone decreased the vessel number in the CAM by 64%, but coadministered with hydrocortisone (156 μg/plug, which alone caused 30% inhibition) failed to potentiate its antiangiogenic effect. Combined with basic fibroblast growth factor (50 ng/plug), SargA (1500 μg/plug) abolished angiogenesis stimulated by this factor in both chick embryo CAM and in subcutaneous (s.c.) Gelfoam plugs implanted in the dorsal skin of Swiss mice (measured as plug hemoglobin content). Repeated s.c. injections of SargA (1.5 or 150 μg per animal per day for 3 days) close to B16F10 melanoma cell tumors in the dorsal skin of mice markedly decreased tumor growth in a dose-related fashion (by 40% and 80% at 2 weeks after the first injection, respectively), without evident signs of toxicity. SargA caused graded inhibitions of migration and viability of cultured B16F10 cells and also displayed antithrombotic activity in human plasma (5 mg/ml increased thrombin time 2.5-fold relative to saline). Thus, SargA exhibits pronounced antiangiogenic as well as antitumoral properties. Although the latter action of SargA might be related to the inhibition of angiogenesis, the polysaccharide also exerts cytotoxic effects on tumor cells. Because of its chemical characteristics and polyanionic constituents, we postulate that the polysaccharide SargA might modulate the activity of heparin-binding angiogenic growth factors.

Keywords

Polysaccharide Angiogenesis Antitumoral activity Sargassum stenophyllum Angiostatic activity 

Notes

Acknowledgements

The authors are indebted to Dr. M.L. Pessatti (Biochemistry and Molecular Biology Laboratory – CTTMar – UNIVALI, Itajaí – SC, Brazil) for providing the preprocessed biomass of the alga and Dr. A.G. Trentin (Laboratory of Cell and Molecular Neurobiology and Hematology– Cell Biology, Embryology and Genetics Department/CCB–UFSC) for providing the B16F10 cells. We are grateful to Dr. G.A. Rae (Pharmacology Department/CCB – UFSC) for his valuable assistance in scientific English as well as his invaluable collaboration in the development of this work.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Paulo Fernando Dias
    • 1
    • 2
  • Jarbas Mota SiqueiraJr
    • 1
  • Luiz Felipe Vendruscolo
    • 3
  • Teresinha de Jesus Neiva
    • 4
  • Antônio Ricardo Gagliardi
    • 5
    • 6
  • Marcelo Maraschin
    • 3
  • Rosa Maria Ribeiro-do-Valle
    • 1
    Email author
  1. 1.Departament of Pharmacology, Biological Sciences Center Block “D”Federal University of Santa CatarinaFlorianópolisBrazil
  2. 2.Department of Cell Biology, Embryology and Genetics – CCBFederal University of Santa CatarinaFlorianópolisBrazil
  3. 3.Plant Morphogenesis and Biochemistry Laboratory CCAFederal University of Santa CatarinaFlorianópolisBrazil
  4. 4.Department of Pharmaceutical Sciences CCSFederal University of Santa CatarinaFlorianópolisBrazil
  5. 5.Department of Physiology, Faculty of Medical Sciences of SantosCentro Universitário Lusíada – UNILUSSantos SPBrazil
  6. 6.Clinical Unit of Dyslipidemias, São Paulo Heart Institute Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo – HC-FMUSPSão PauloBrazil

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