Cancer Chemotherapy and Pharmacology

, Volume 56, Issue 1, pp 70–74 | Cite as

In vivo antitumor activity by 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone in a solid human carcinoma xenograft model

  • Chun-Lin Ye
  • Jian-Wen Liu
  • Dong-Zhi Wei
  • Yan-Hua Lu
  • Feng Qian
Original Article


Previously we have shown that 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC), which is isolated from the buds of Cleistocalyx operculatus, significantly inhibits the growth of human liver cancer SMMC-7721 cells and is able to induce apoptosis of SMMC-7721 cells in vitro. Here we report the antitumor effects of DMC in vivo, using a solid human tumor xenograft mouse model using human liver cancer SMMC-7721 cells. The average tumor weights in the control group and in mice injected with 150 mg/kg DMC were 1.42±0.11 g and 0.59±0.12 g, respectively. Flow cytometric analysis of the tumor cell population demonstrated an aneuploid peak (representing 33.60±0.80% of the total in mice injected with 150 mg/kg DMC). To our knowledge, this is the first time that chalcone compounds have been applied to a human tumor xenograft model.


Cleistocalyx operculatus Flavonoids Tumor Acute toxicity Xenograft 


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Chun-Lin Ye
    • 1
  • Jian-Wen Liu
    • 1
  • Dong-Zhi Wei
    • 1
  • Yan-Hua Lu
    • 1
  • Feng Qian
    • 1
  1. 1.State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry East China University of Science and TechnologyShanghaiPeople’s Republic of China

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