Advertisement

Annals of Hematology

, Volume 80, Issue 11, pp 639–646 | Cite as

Use of pathology-specific peripheral blood CD34 thresholds to predict leukapheresis CD34 content with optimal accuracy: a bicentric analysis of 299 leukaphereses

  •  I. Dobo
  •  N. Robillard
  •  D. Pineau
  •  F. Geneviève
  •  N. Piard
  •  M.-J. Rapp
  •  M. Boasson
  •  M. Zandecki
  •  S. Hermouet
Original Article

Abstract.

CD34+ cell counts in peripheral blood (PB) and corresponding numbers of CD34+ cells and colony-forming units-granulocyte/macrophage (CFU-GM) in 299 leukapheresis products of 209 patients undergoing PB progenitor cell (PBPC) mobilization for autologous transplantation in two different centers were analyzed and compared according to diagnosis: non-Hodgkin lymphoma (NHL, 94 leukaphereses), multiple myeloma (MM, 75), Hodgkin's disease (HD, 37), solid tumors (35), and chronic myeloid leukemia (CML, 32). Without separating disease entities, correlations between PB CD34+ cell counts and leukapheresis content of CD34+ cells (r>0.83, P<0.01) and CFU-GM (r>0.81, P<0.01) were excellent. In both centers, a PB CD34 threshold ensuring a leukapheresis yield >106 CD34/kg was determined. This threshold was higher in center 1 than in center 2, and its predictive accuracy (91.4%, i.e., prediction correct 91.4% of the time) was significantly lower than in center 2 (98.4%, P=0.02). When data were analyzed by pathology, PB CD34+ cell counts and leukapheresis content of CD34+ cells and CFU-GM remained well correlated, and in both centers PB CD34 thresholds predictive of a yield >106 CD34/kg per leukapheresis could be determined for each pathology. For most patients, pathology-specific PB CD34 thresholds could be obtained directly from the equation of the PB CD34/leukapheresis CD34 correlation curve; they varied depending on both pathology and center (range: 7–20×106 CD34/l). Pathology-specific thresholds predicted a leukapheresis yield ≥106 CD34/kg accurately 100% of the time for MM patients in center 2 and HD and solid tumor patients of both centers, resulting in overall rates of accurate prediction of sufficient graft CD34 content of 96.6% in center 1 and 98.9% in center 2.

Peripheral blood CD34 PBPC CD34 Colony-forming units-granulocyte/macrophage (CFU-GM) Leukapheresis yield Pathology-specific peripheral blood CD34 thresholds 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  I. Dobo
    • 1
  •  N. Robillard
    • 2
  •  D. Pineau
    • 2
  •  F. Geneviève
    • 1
  •  N. Piard
    • 3
  •  M.-J. Rapp
    • 4
  •  M. Boasson
    • 5
  •  M. Zandecki
    • 1
  •  S. Hermouet
    • 2
  1. 1.Laboratoire d'Hématologie du Centre Hospitalier Universitaire (CHU) d'Angers, France
  2. 2.Laboratoire d'Hématologie du CHU de Nantes, Institut de Biologie, CHU Nantes, 9 Quai Moncousu, 44093 Nantes Cedex 01, France
  3. 3.Etablissement de Transfusion Sanguine de Maine-et-Loire, Angers, France
  4. 4.Service d'Hématologie Clinique du CHU de Nantes, France
  5. 5.Service des Maladies du Sang du CHU d'Angers, France

Personalised recommendations