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Vascular density and inflammatory infiltrate in primary oral squamous cell carcinoma and after allogeneic hematopoietic stem cell transplantation

  • Roberto Tamma
  • Luisa Limongelli
  • Eugenio Maiorano
  • Domenico Pastore
  • Eliano Cascardi
  • Angela Tempesta
  • Paola Carluccio
  • Mauro G. Mastropasqua
  • Saverio Capodiferro
  • Claudia Covelli
  • Monica Pentenero
  • Tiziana Annese
  • Gianfranco Favia
  • Giorgina Specchia
  • Domenico Ribatti
Original Article

Abstract

Hematopoietic stem cell transplantation (HSCT) recipients have been reported to have an increased risk of chronic graft versus host disease (cGVHD) and hematological and solid cancers. Oral manifestations are the first signs of cGVHD observed in the majority of patients, and oropharyngeal cancer is the most frequent secondary malignancy occurred after HSCT. In this study, we have evaluated the inflammatory infiltrate cell content and correlated with the vascular density in patients affected by primary oral squamous cell carcinoma (OSCC) from previous healthy controls and OSCC after cGVHD. Results have demonstrated that patients with OSCC after GVHD show a more consistent inflammatory infiltrate as compared with the OSCC ones. In detail, the inflammatory background composed of CD3-positive T cells, tryptase-positive mast cells, CD31-positive endothelial cells, and CD68-positive macrophages may be more pronounced in the setting of GVHD + OSCC than in the control group. By contrast, CD20-positive B cells and CD1a-positive dendritic cells were more abundant in the latter population. Finally, a positive correlation was found as between vascular density and inflammatory cell infiltration in both GVHD + OSCC and OSCC groups. Overall, these results confirm the role played by immune cells in enhancing tumor progression and angiogenesis and suggest a potential therapeutic strategy involving inhibition of recruitment of immune cells to the tumor microenvironment and blockade of pro-tumoral effects and pro-angiogenic functions.

Keywords

Allogeneic hematopoietic stem cell transplantation Chronic graft versus host disease Inflammatory infiltrate Primary oral squamous cell carcinoma Vascular density 

Notes

Author Contributions

All authors have seen and approved the manuscript being submitted.

Funding information

This work was supported in part by Fellowship FIRC-AIRC 1-year fellowship “Laura Bassi” id. 20879 to TA.

Compliance with ethical standards

Full ethical approval and signed informed consent from individual patients were obtained to conduct the study. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1964 and later versions.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Roberto Tamma
    • 1
  • Luisa Limongelli
    • 2
  • Eugenio Maiorano
    • 3
  • Domenico Pastore
    • 4
  • Eliano Cascardi
    • 3
  • Angela Tempesta
    • 2
  • Paola Carluccio
    • 4
  • Mauro G. Mastropasqua
    • 3
  • Saverio Capodiferro
    • 2
  • Claudia Covelli
    • 3
  • Monica Pentenero
    • 5
  • Tiziana Annese
    • 1
  • Gianfranco Favia
    • 2
  • Giorgina Specchia
    • 4
  • Domenico Ribatti
    • 1
  1. 1.Department of Basic Medical Sciences, Neurosciences, and Sensory OrgansUniversity of Bari Medical SchoolBariItaly
  2. 2.Department of Interdisciplinary Medicine, Section of OdontostomatologyUniversity of Bari Medical SchoolBariItaly
  3. 3.Department of Emergency and Transplantation, Pathology SectionUniversity of Bari Medical SchoolBariItaly
  4. 4.Department of Emergency and Transplantation, Hematology SectionUniversity of Bari Medical SchoolBariItaly
  5. 5.Oral Medicine and Oral Oncology Unit, Department of OncologyUniversity of Turin Medical SchoolTurinItaly

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