Genotyping of 22 blood group antigen polymorphisms and establishing a national recipient registry in the Korean population
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It is often difficult for standard blood banks in Korea to supply adequate amounts of blood for patients with rare phenotype. Moreover, the definition of a blood in need is ambiguous, and much remains to be learned. In this study, we determined the prevalence of various red blood cell (RBC) antigens from a donor viewpoint and estimated the demand for specific antigen-negative blood from a patient viewpoint. Our data will aid the establishment of a Rare Blood Program in Korea (KRBP). RBC genotyping of 419 blood donors was performed using a Lifecodes RBC/RBC-R typing kit (Immucor, Norcross, GA). A national recipient registry website has been established. Each hospital-based blood bank voluntarily enters data on antibodies detected and identified and the outcomes of specific antigen testing. We calculated the availabilities of specific antigen-negative blood components based on these registry data and predicted the prevalence of RBC antigens via RBC genotyping. The prevalences of various RBC antigens in the D-negative population were determined for the first time, and the Cartwright, Scianna, Dombrock, Colton, Landsteiner-Wiener, Cromer, and Knops blood group systems were identified. The availabilities of specific antigen-negative units differed when calculations were based on serotyping or genotyping, especially in the D-negative group. Data on the prevalences of various blood antigens are essential for estimating the availabilities of blood components that are appropriate for use by patients expressing relevant antibodies. Then, blood banks would be able to efficiently supply safe blood products.
KeywordsRBC genotyping RhD negatives Luminex
This study was supported by research fund for “frequency investigation of rare blood groups in Koreans and management system development of associated blood components (2011E3400300 and 08-2012-010) and establishment and operation of the Korean Rare Blood Program (KRBP) for personalized transfusion (20121206077-00 and 20140506667-00)” from Korea Centers for Disease Control and Prevention.
Compliance with ethical standards
Conflict of interest
The authors have disclosed no conflicts of interest.
- 1.Fung MK, Grossman BJ, Hillyer CD (2014) Technical manual. American Association of Blood Banks, BethesdaGoogle Scholar
- 7.Kim IT, Suh IB, Ma KR, Lim CS, Kim YK, Lee KN (2003) The genotyping of Kell, Duffy, and Kidd System in Korean. Korean J Blood Transfus 14:9–19Google Scholar
- 9.Lee SY (1975) Possible problems on the blood-typological studies of Korean caused by inter-racial marriages with Caucasians. Korean J Hematol 10:33–39Google Scholar
- 11.Chung Y, Hong YJ, Hwang SM, Kim TS, Park KU, Song J, Han KS (2014) Probability of obtaining specific antigen-negative blood units through antigen testing in Korean Medical Institutes. Korean J Blood Transfus 25:34–40Google Scholar
- 13.Hashmi G, Shariff T, Zhang Y, Cristobal J, Chau C, Seul M, Vissavajjhala P, Baldwin C, Hue-Roye K, Charles-Pierre D, Lomas-Francis C, Reid ME (2007) Determination of 24 minor red blood cell antigens for more than 2000 blood donors by high-throughput DNA analysis. Transfus 47:736–747CrossRefGoogle Scholar
- 14.Ok SJ, Kim SY, Kim IS, Lee EY, Kim HH (2013) Eleven years’ experience with unexpected antibody screening tests including a Di(a) cell in transfusion candidates. Korean J Blood Transfus 24:64–70Google Scholar
- 16.Choi S, Kim SI, Cho HI (1984) Study on gene frequencies of blood groups in Koreans. Korean J Hematol 19:63–75Google Scholar
- 17.Reesink HW, Engelfriet CP, Schennach H, Gassner C, Wendel S, Fontão-Wendel R, de Brito MA, Sistonen P, Matilainen J, Peyrard T, Pham BN, Rouger P, Le Pennec PY, Flegel WA, von Zabern I, Lin CK, Tsoi WC, Hoffer I, Barotine-Toth K, Joshi SR, Vasantha K, Yahalom V, Asher O, Levene C, Villa MA, Revelli N, Greppi N, Marconi M, Tani Y, Folman CC, de Haas M, Koopman MMW, Beckers E, Gounder DS, Flanagan P, Wall L, Aranburu Urtasun E, Hustinx H, Niederhauser C, Flickinger C, Nance SJ, Meny GM (2008) Donors with a rare pheno (geno) type. Vox Sang 95:236–253CrossRefPubMedGoogle Scholar