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Annals of Hematology

, Volume 93, Issue 2, pp 309–315 | Cite as

Antiplatelet antibodies detected by the MAIPA assay in newly diagnosed immune thrombocytopenia are associated with chronic outcome and higher risk of bleeding

  • David Grimaldi
  • Florence Canouï-Poitrine
  • Laure Croisille
  • Ketty Lee
  • Françoise Roudot-Thoraval
  • Laetitia Languille
  • Medhi Khellaf
  • Marc Michel
  • Bertrand Godeau
  • Philippe BierlingEmail author
Original Article

Abstract

Immune thrombocytopenia (ITP) results in part from the presence of platelet antibodies, which can be demonstrated by the Monoclonal Antibody-Specific Immobilization of Platelet Antigens (MAIPA) assay. The aim of our study was to correlate the presence of antiplatelet autoantibodies and the natural history of ITP. We performed a retrospective, single-center study of 108 adults with newly diagnosed ITP who had indirect MAIPA assay performed at disease onset. Chronic ITP was defined by the presence of thrombocytopenia after 1 year. Bleeding diathesis was evaluated with a bleeding score. At baseline, patients with a positive indirect MAIPA have a greater bleeding score than patients with negative MAIPA assay [median (interquartile) = 8 (6–12) vs 2 (0–6), p = 0.002]. Patients with a positive indirect MAIPA also had a higher rate of chronic ITP (92.9 vs 68.7 %, p = 0.06). In multivariate analysis, a positive indirect MAIPA result and a platelet count at onset ≥10 × 109/L remained independently associated with chronic ITP [adjusted OR (aOR) = 8.01; 95 % confidence interval (CI), 0.98–66.6; p = 0.05 and aOR = 3.09; 95 % CI, 1.18–8.10; p = 0.02, respectively]. Furthermore, when we analyzed together the results of direct (n = 41) and indirect MAIPA, the same results were observed. Thus, indirect MAIPA positivity at disease onset is associated with more severe hemorrhage and predicts a chronic course in adult ITP patients. MAIPA assay could be useful in the management of ITP patients when it is performed at diagnosis.

Keywords

Immune thrombocytopenia MAIPA assay Chronic ITP Platelet autoantibodies 

Notes

Acknowledgment

The authors would like to gratefully thank Aurélie Le Thuaut for her technical help in statistical analysis. DG, BG and PB designed the study. BG, PB, MK, MM enrolled the patients. DG and LL recorded the data of the study. FCP and FRT performed the statistical analysis. KL, LC and PB performed MAIPA test. DG, FCP, MK, MM, BG and PB wrote the manuscript.

Supplementary material

277_2013_1855_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 16 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • David Grimaldi
    • 1
  • Florence Canouï-Poitrine
    • 2
    • 3
  • Laure Croisille
    • 1
    • 4
  • Ketty Lee
    • 1
    • 4
  • Françoise Roudot-Thoraval
    • 2
    • 3
  • Laetitia Languille
    • 1
  • Medhi Khellaf
    • 1
  • Marc Michel
    • 1
  • Bertrand Godeau
    • 1
  • Philippe Bierling
    • 1
    • 4
    Email author
  1. 1.Service de Médecine interne, Centre de référence des cytopénies auto-immunes, hôpital Henri Mondor, AP-HPUniversité Paris EstCréteilFrance
  2. 2.Université Paris EstCréteilFrance
  3. 3.AP-HP, Service de santé publiqueHôpital Henri MondorCréteilFrance
  4. 4.Etablissement Francais du SangHopital Henri MondorCreteilFrance

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