Annals of Hematology

, Volume 91, Issue 10, pp 1623–1631 | Cite as

Decreased indoleamine 2,3-dioxygenase expression in dendritic cells and role of indoleamine 2,3-dioxygenase-expressing dendritic cells in immune thrombocytopenia

  • Shu-qian Xu
  • Chun-yan Wang
  • Xiao-juan Zhu
  • Xiao-yuan Dong
  • Yan Shi
  • Jun Peng
  • Ping Qin
  • Jian-zhi Sun
  • Chengshan Guo
  • Heyu Ni
  • Ming Hou
Original Article


Indoleamine 2,3-dioxygenase (IDO) expression in dendritic cells (DCs) can induce or maintain peripheral immune tolerance. Impaired IDO-mediated tryptophan catabolism has been observed in autoimmune diseases. In order to investigate the effects of IDO-mediated tryptophan catabolism and IDO-expressing DCs in immune thrombocytopenia, the concentrations of kynurenine were detected by high-pressure liquid chromatography. The expressions of IDO were analyzed by flow cytometry and western blot analysis. The effects of IDO+ DCs stimulated with CTLA-4-Ig on T cells proliferation and activation, lymphocyte apoptosis, and Tregs were measured by flow cytometry. We found that the expression of IDO in DCs of immune thrombocytopenia (ITP) patients was significantly decreased. CTLA-4-Ig significantly increased the expression of functional IDO in DCs of ITP patients. IDO+ DCs stimulated with CTLA-4-Ig suppressed T cells proliferation and activation, promoted lymphocyte apoptosis, and increased the percentage of Tregs. These results suggest that decreased IDO expression in DCs may play a critical role in ITP. CTLA-4-Ig successfully corrected the disorder of IDO expression in ITP. IDO+ DCs stimulated with CTLA-4-Ig inhibited immune responses by an IDO-dependent mechanism. Increasing the expression and activity of IDO in DCs might be a promising therapeutic approach for ITP.


Indoleamine 2,3-dioxygenase CTLA-4-Ig Dendritic cells Immune thrombocytopenia 

Supplementary material

277_2012_1451_MOESM1_ESM.doc (40 kb)
Supplementary Table 1Clinical characteristics of ITP patients. (DOC 40 kb)


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Shu-qian Xu
    • 1
  • Chun-yan Wang
    • 1
    • 2
  • Xiao-juan Zhu
    • 1
    • 3
  • Xiao-yuan Dong
    • 1
  • Yan Shi
    • 1
  • Jun Peng
    • 1
    • 4
  • Ping Qin
    • 1
  • Jian-zhi Sun
    • 1
  • Chengshan Guo
    • 5
  • Heyu Ni
    • 6
  • Ming Hou
    • 1
    • 4
  1. 1.Hematology Oncology Centre, Qilu HospitalShandong UniversityJinanChina
  2. 2.Department of General MedicineSecond Hospital of Shandong UniversityJinanChina
  3. 3.Department of General MedicineProvincial Hospital affiliated to Shandong UniversityJinanChina
  4. 4.Key Laboratory of Cardiovascular Remodeling and Function ResearchChinese Ministry of Education and Chinese Ministry of HealthJinanChina
  5. 5.Department of HematologySecond Hospital of Shandong UniversityJinanChina
  6. 6.Canadian Blood Services and Toronto Platelet Immunobiology Group and Department of Laboratory Medicine and Pathobiology, Keenan Research Centre in the Li Ka Shing Knowledge Institute of St. Michael’s HospitalUniversity of TorontoTorontoCanada

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