Annals of Hematology

, 85:291 | Cite as

MTHFR 677 (C→T) polymorphism is not relevant for prognosis or therapy-associated toxicity in pediatric NHL: results from 484 patients of multicenter trial NHL-BFM 95

  • Kathrin Seidemann
  • Marion Book
  • Martin Zimmermann
  • Ulrike Meyer
  • Karl Welte
  • Martin Stanulla
  • Alfred Reiter
Original Article

Abstract

We analyzed the relationship of genetic variation within the methylenetetrahydrofolate reductase gene (MTHFR 677 C→T) with clinical characteristics, outcome, and therapy-related toxicity in pediatric non-Hodgkin’s lymphoma (NHL) in our multicenter trial NHL-BFM 95. In this trial, high-dose methotrexate (MTX) infusion regimens were randomized (4- vs 24-h infusion) in patients with B-cell lymphoma; MTX was applied as 24-h infusion in all patients with lymphoblastic lymphoma and anaplastic large cell lymphoma. Toxicity data were collected per patient and therapy course according to National Cancer Institute Common Toxicity Criteria (NCI-CTC). The genotypes in 484 pediatric patients were distributed as follows: MTHFR 677 CC, 206 patients (42.6%); MTHFR 677 CT, 214 patients (44.2%); and MTHFR 677 TT, 64 patients (13.2%). Lymphoblastic lymphoma was significantly associated with homozygosity for the MTHFR 677 T allele. No association of MTHFR 677 genotype with clinical characteristics (sex, age, and tumor stage), outcome, or therapy-related toxicity could be detected. Therefore, we conclude that the MTHFR 677 C→T polymorphism does not appear to influence outcome or therapy-associated toxicity in pediatric patients with NHL treated on BFM protocols.

Keywords

MTHFR Polymorphism Toxicity Pediatric NHL BFM 

Notes

Acknowledgements

We thank E. Odenwald for expert work in cytological diagnosis and U. Meyer for excellent data management. We especially thank all doctors and nurses in participating hospitals for their continuous care for sick children and their excellent cooperation with study center NHL-BFM. Supported by the Deutsche Krebshilfe, Bonn (grant no. M 109/91/Re1) and by the Verein zur Förderung der Behandlung krebskranker Kinder Hannover e.V.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Kathrin Seidemann
    • 1
  • Marion Book
    • 1
  • Martin Zimmermann
    • 1
  • Ulrike Meyer
    • 2
  • Karl Welte
    • 1
  • Martin Stanulla
    • 1
  • Alfred Reiter
    • 2
  1. 1.Department of Pediatric Hematology and OncologyHannover Medical SchoolHannoverGermany
  2. 2.NHL-BFM Study Center, Department of Pediatric Hematology and OncologyUniversity Children’s Hospital, Justus Liebig University GiessenGiessenGermany

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