Irrigation Science

, Volume 30, Issue 4, pp 247–257 | Cite as

Irrigation control of cowpea plants using the measurement of leaf thickness under greenhouse conditions

  • Hans-Dieter SeeligEmail author
  • Richard J. Stoner
  • James C. Linden
Original Paper


In this study, the thickness of cowpea leaves was measured with high data- and time-resolution, and the dynamics of leaf thickness was subsequently used as an input parameter for automated irrigation control at the greenhouse level. Under non-stressful environmental conditions, leaf thickness showed only minor diurnal and almost no nocturnal fluctuations. In an extreme water deficit stress scenario, leaf thickness decreased dramatically by as much as 45% within a short period of time. In a more realistic situation, leaf thickness was kept fairly constant for several days, but decreased substantially when water deficit stress became too severe for the plants to cope with any longer. This characteristic collapse of leaf thickness was used as an input parameter for the automated initiation of irrigation. Upon automated irrigation, plants re-established their nominal leaf thickness quickly and kept this leaf thickness constant for several days, until signaling the need for the next irrigation by a subsequent decrease of leaf thickness. By using the measurement of leaf thickness for irrigation control, between 25 and 45% of irrigation water could be conserved compared with a typical timed irrigation schedule.


Leaf Thickness Water Deficit Stress Irrigation Event Daily Fluctuation Irrigation Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by the National Science Foundation, award-number 0712605. We also would like to thank Tom Lemieux of the University of Colorado’s greenhouse for his advice, Dr. Ken Knutson of the College of Agricultural Sciences at Colorado State University for his review comments, and the anonymous journal-reviewers for their comments.


  1. Alarcon JJ, Malone M (1994) Substantial hydraulic signals are triggered by leaf-biting insects in tomato. J Exp Bot 45:953–957CrossRefGoogle Scholar
  2. Bachmann F (1922) Studien über die Dickenänderung von Laubblättern. Jahrb wiss Bot 61:372–429Google Scholar
  3. Búrquez A (1987) Leaf thickness and water deficit in plants: a tool for field studies. J Exp Bot 38:109–114CrossRefGoogle Scholar
  4. Carpenter SB, Smith ND (1979) Variations in shade leaf thickness among urban trees growing in metropolitan Lexington, Kentucky. Castanea 44:94–98Google Scholar
  5. Carpenter SB, Smith ND (1981) A comparative study of leaf thickness among southern Appalachian hardwoods. Can J Bot 59:1393–1396CrossRefGoogle Scholar
  6. Chaney WR (1970) A device for the continuous monitoring of changes in leaf thickness. Forest Sci 16:56Google Scholar
  7. Cooper RL, Ware JV, Cass DD (2004) Leaf thickness of Salix spp. (Salicaceae) from the Athabasca sand dunes of northern Saskatchewan, Canada. Can J Bot 82:1682–1686CrossRefGoogle Scholar
  8. Danson FM, Steven MD, Malthus TJ, Clark JA (1992) High spectral resolution data for determining leaf water content. Int J Remote Sens 13:461–470CrossRefGoogle Scholar
  9. Esau K (1977) Anatomy of seed plants. Wiley, New YorkGoogle Scholar
  10. Friend DJ (1961) Control of chlorophyll accumulation in leaves of Marquis wheat by temperature and light intensity. II. Chlorophyll contents relative to leaf area and thickness. Physiol Plant 14:28–39CrossRefGoogle Scholar
  11. Gausman HW (1974) Leaf reflectance of near-infrared. Photogramm Eng 40:183–191Google Scholar
  12. Gausman HW, Allen WA, Cardenas R, Richardson AJ (1970) Relation of light reflectance to historical and physical evaluations of cotton leaf maturity. Appl Opt 9:545–552PubMedCrossRefGoogle Scholar
  13. Hanba YT, Miyazawa SI, Terashima I (1999) The influence of leaf thickness on the CO2 transfer conductance and leaf stable carbon isotope ratio for some evergreen tree species in Japanese warm-temperature forests. Funct Ecol 13:632–639CrossRefGoogle Scholar
  14. Heilman MD, Gonzales CL, Swanson WA, Rippert WJ (1968) Adaptation of a linear transducer for measuring leaf thickness. Agron J 60:578CrossRefGoogle Scholar
  15. Kadoya K (1978) Studies on hydrophysiological rhythms of citrus trees. III. Effect of soil-temperature on cyclic fluctuations of leaf thickness. J Jpn Soc Hortic Sci 47:167–171CrossRefGoogle Scholar
  16. Li J, Yang J, Fei P, Song J, Li D, Ge C, Chen W (2009) Responses of rice leaf thickness, SPAD readings and chlorophyll a/b ratios to different nitrogen supply rates in paddy field. Field Crops Res 114:426–432CrossRefGoogle Scholar
  17. Malone M (1992) Kinetics of wound-induced hydraulic signals and variation potentials in wheat seedlings. Planta 187:505–510CrossRefGoogle Scholar
  18. Malone M, Alarcon JJ (1994) Only xylem-borne factors can account for systemic wound signaling in the tomato plant. Planta 196:740–746CrossRefGoogle Scholar
  19. Marenco RA, Antezana-Vera SA, Nascimento HCS (2009) Relationship between specific leaf area, leaf thickness, leaf water content and SPAD-502 readings in six Amazonian tree species. Photosynthetica 47:184–190CrossRefGoogle Scholar
  20. McBurney T (1992) The relationship between leaf thickness and plant water potential. J Exp Bot 43:327–335CrossRefGoogle Scholar
  21. McCain DC, Croxdale J, Markley JL (1988) Water is allocated differently in chloroplasts in sun and shade leaves. Plant Physiol 86:16–18PubMedCrossRefGoogle Scholar
  22. Meidner H (1952) An instrument for the continuous determination of leaf thickness changes in the field. J Exp Bot 3:319–325CrossRefGoogle Scholar
  23. Niinemets U (1999) Components of leaf dry mass per area–thickness and density–alter leaf photosynthetic capacity in reverse directions in woody plants. New Phytol 144:35–47CrossRefGoogle Scholar
  24. Nobel PS, Hartsock TL (1981) Development of leaf thickness for Plectranthus parviflorus–influence of photosynthetically active radiation. Physiol Plant 51:163–166CrossRefGoogle Scholar
  25. Rozema J, Arp W, Van Diggelen J, Kok E, Letschert J (1987) An ecophysiological comparison of measurements of the diurnal rhythm of the leaf elongation and changes of the leaf thickness of salt-resistant dicotyledonae and monocotyledonae. J Exp Bot 38:442–453CrossRefGoogle Scholar
  26. Semerdjieva SI, Phoenix GK, Hares D, Gwynn-Jones D, Callaghan TV, Sheffield E (2003) Surface morphology, leaf and cuticle thickness of four dwarf shrubs from a sub-Artic heath following long-term exposure to enhanced levels of UV-B. Physiol Plant 117:289–294CrossRefGoogle Scholar
  27. Sharon Y, Bravdo B-A (1996) Irrigation control for citrus according to the diurnal cycling of leaf thickness. Proceedings of the international conference on water & irrigation, Tel Aviv, Israel, pp 273–283Google Scholar
  28. Sinclair TR (1968) Pathway of solar radiation through leaves. M.S. thesis, Purdue University, Lafayette, INGoogle Scholar
  29. Sinclair TR, Ludlow MM (1985) Who taught plants thermodynamics? The unfulfilled potential of plant water potential. Aust J Plant Physiol 12:213–217CrossRefGoogle Scholar
  30. Syvertsen J, Levy Y (1982) Diurnal changes in citrus leaf thickness, leaf water potential, and leaf to air temperature difference. J Exp Bot 33:783–789CrossRefGoogle Scholar
  31. Taiz L, Zeiger E (eds) (2006) Plant physiology, 4th edn. Sinauer Associates, SunderlandGoogle Scholar
  32. Terashima I, Hanba YT, Tazoe Y, Vyas P, Yano S (2006) Irradiance and phenotype: comparative eco-development of sun and shade leaves in relation to photosynthetic CO2 diffusion. J Exp Bot 57:343–354PubMedCrossRefGoogle Scholar
  33. Thomas JR, Namken LN, Oerther GG, Brown RG (1971) Estimating leaf water content by reflectance measurements. Agron J 63:845–847CrossRefGoogle Scholar
  34. Tyree MT, Cameron SI (1977) A new technique for measuring oscillatory and diurnal changes in leaf thickness. Can J For Res 7:540–544CrossRefGoogle Scholar
  35. Vile D, Garnier É, Shipley B, Laurent G, Navas M-L, Roumet C, Lavorel S, Diaz S, Hodgson JG, LLoret F, Midgley GF, Poorter H, Rutherford MC, Wilson PJ, Wright IJ (2005) Specific leaf area and dry matter content estimate thickness in laminar leaves. Ann Bot 96:1129–1136PubMedCrossRefGoogle Scholar
  36. Vogelmann TC, Bornman JF, Josserand S (1989) Photosynthetic light gradients and spectral regime within leaves of Medicago sativa. Philos Trans R Soc Lond Ser B 323:411–421CrossRefGoogle Scholar
  37. White JW, Montes-R C (2005) Variation in parameters related to leaf thickness in common bean (Phaseolus vulgaris L.). Field Crops Res 91:7–21CrossRefGoogle Scholar
  38. Yun JI, Taylor SE (1986) Adaptive implications of leaf thickness for sun- and shade-grown Abutilon theophrasti. Ecology 67:1314–1318CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Hans-Dieter Seelig
    • 1
    Email author
  • Richard J. Stoner
    • 2
  • James C. Linden
    • 3
  1. 1.Department of Aerospace Engineering SciencesUniversity of Colorado at BoulderBoulderUSA
  2. 2.Agrihouse Inc.BerthoudUSA
  3. 3.Department of Microbiology, Immunology, and PathologyColorado State UniversityFort CollinsUSA

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