The Novel X-Ray Visible Zein-Based Non-adhesive Precipitating Liquid Embolic HEIE1_2017: An Exploratory Study

  • Dominik F. Vollherbst
  • Thuy D. Do
  • Manfred Jugold
  • Viktoria Eichwald
  • Stephan Macher-Göppinger
  • Philippe L. Pereira
  • Martin Bendszus
  • Markus A. Möhlenbruch
  • Götz M. Richter
  • Hans U. Kauczor
  • Christof M. SommerEmail author
Laboratory Investigation



To investigate the novel zein-based non-adhesive precipitating liquid embolic HEIE1_2017.

Materials and Methods

Zein-based liquid embolics are an own class of embolization material. In this study, HEIE1_2017, a novel zein-based liquid embolic, was investigated. Visibility was assessed in vitro in CT and MRI phantoms, embolization characteristics were assessed in vivo in the kidneys of 12 pigs. Components of HEIE1_2017 were zein as occlusion material, ethanol as solvent, and iodized oil as radiopaque material. HEIE1_2017 was used in pure (HEI-PURE) and manually modified (HEI-MOD) form and compared with 6% ethylene vinyl alcohol copolymer (EVOH). Different radiological methods (CT, MRI, DSA, cone-beam CT, and micro-CT) and histopathologic analyses were applied to compare visibility and vascular occlusion patterns.


In CT phantoms, all embolics were definitely visible as hyperdense materials. In MRI phantoms, signal-to-noise ratio was highest for HEI-PURE, followed by HEI-MOD and EVOH. In all kidneys, embolization procedures were technically successful and without complications. In DSA, all embolics were definitely visible during and after embolization. Only EVOH caused substantial artifacts in cone-beam CT and CT. In micro-CT and histopathology, HEI-PURE showed a homogeneous occlusion from segmental arteries to glomerular capillaries. HEI-MOD demonstrated the deepest vascular penetration (up to the level of peritubular capillaries), but with an inhomogeneous distribution. For EVOH, there was inhomogeneous vascular occlusion from segmental arteries to glomerular capillaries.


HEIE1_2017 is a promising novel zein-based liquid embolic. Further preclinical and clinical studies with higher case numbers and long-term follow-up are needed to further assess the value of this embolic material.


Embolization Liquid embolic Ethibloc Onyx Histoacryl 


Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

270_2019_2179_MOESM1_ESM.doc (43 kb)
Supplementary material 1 (DOC 43 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2019

Authors and Affiliations

  • Dominik F. Vollherbst
    • 1
    • 2
  • Thuy D. Do
    • 2
  • Manfred Jugold
    • 3
  • Viktoria Eichwald
    • 3
  • Stephan Macher-Göppinger
    • 4
  • Philippe L. Pereira
    • 6
  • Martin Bendszus
    • 1
  • Markus A. Möhlenbruch
    • 1
  • Götz M. Richter
    • 5
  • Hans U. Kauczor
    • 2
  • Christof M. Sommer
    • 2
    • 5
    Email author
  1. 1.Department of NeuroradiologyHeidelberg University HospitalHeidelbergGermany
  2. 2.Clinic of Diagnostic and Interventional RadiologyHeidelberg University HospitalHeidelbergGermany
  3. 3.German Cancer Research CenterHeidelbergGermany
  4. 4.Institute of PathologyMainz University HospitalMainzGermany
  5. 5.Clinic of Diagnostic and Interventional RadiologyKlinikum StuttgartStuttgartGermany
  6. 6.Clinic for Radiology, Minimally-invasive Therapies and Nuclear MedicineSLK Kliniken Heilbronn GmbHHeilbronnGermany

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