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Monitoring of Thermal-Induced Changes in Liver Stiffness During Controlled Hyperthermia and Microwave Ablation in an Ex Vivo Bovine Model Using Point Shear Wave Elastography

  • Laura Crocetti
  • Francesca Calcagni
  • Giulia Gherarducci
  • Nevio Tosoratti
  • Claudio Amabile
  • Francesco Paolo Tarantino
  • Irene Bargellini
  • Simone Cassarino
  • Roberto Cioni
  • Davide Caramella
Laboratory Investigation

Abstract

Purpose

To investigate liver stiffness changes—evaluated by point shear wave elastography (pSWE)—in controlled hyperthermia and microwave ablation (MWA) in an ex vivo animal model.

Materials and Methods

Five samples of ex vivo bovine liver were uniformly heated to temperatures ranging from 40 to 100 °C. B-mode ultrasound imaging and pSWE were acquired simultaneously, and shear wave velocity (SWV) was measured in a region of interest (ROI). The threshold value of SWV at 60 °C (avg60) was identified. Subsequently, MWA was performed in 11 liver samples at 60 W until avg60 + 0.5 m/s was reached. SWV was measured in ROIs at 10–40 mm from the antenna feed. The correlation of mean values of SWV with location (within, border, or outside necrotic area) at gross pathology was evaluated.

Results

In controlled hyperthermia experiments, a steep transition in liver stiffness was observed at 63.0 ± 2.4 °C (SWV 3.54 ± 0.68 m/s). Avg60 was of 2.5 m/s. In 8/9 MWA experiments, interrupted when SWV of 3 m/s was measured, the ROI was at the inner side of the necrotic area border at pathology (accuracy 89%). No correlation between SWV values for outside, border, and within necrosis could be identified.

Conclusions

pSWE can provide a velocity threshold predictive of the presence of coagulation necrosis during MWA in ex vivo liver model. However, pSWE is not able to reliably capture changes in stiffness within, at the border, and outside the necrotic zone in this experimental model.

Keywords

Thermal ablation Shear wave velocity Elastosonography Liver tissue Microwave Stiffness 

Notes

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

270_2018_2152_MOESM1_ESM.docx (1.5 mb)
Supplementary material 1 (DOCX 1567 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2019

Authors and Affiliations

  • Laura Crocetti
    • 1
  • Francesca Calcagni
    • 1
  • Giulia Gherarducci
    • 1
  • Nevio Tosoratti
    • 2
  • Claudio Amabile
    • 2
  • Francesco Paolo Tarantino
    • 1
  • Irene Bargellini
    • 1
  • Simone Cassarino
    • 2
  • Roberto Cioni
    • 1
  • Davide Caramella
    • 1
  1. 1.Department of Diagnostic and Interventional RadiologyCisanello University HospitalPisaItaly
  2. 2.R&D Unit, H.S. Hospital Service SpARomeItaly

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