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CardioVascular and Interventional Radiology

, Volume 36, Issue 2, pp 498–504 | Cite as

Short-Term Effects of Ankaferd Hemostat for Renal Artery Embolization: An Experimental Study

  • Orhan OzbekEmail author
  • Kadir Acar
  • Osman Koc
  • Kadir Saritas
  • Hatice Toy
  • Yalcin Solak
  • Seda Ozbek
  • Ahmet Kucukapan
  • Ibrahim Guler
  • Abduzhappar Gaipov
  • Suleyman Turk
  • Ibrahim Celaleddin Haznedaroglu
Laboratory Investigation

Abstract

Purpose

Renal artery embolization (RAE) is a minimally invasive therapeutic technique that is utilized in a number of disorders. Ankaferd is a novel hemostatic agent with a new mechanism of action independent of clotting factors. We used Ankaferd for RAE in a sheep model.

Methods

Seven adult female sheep were included in the study. Selective renal arteriogram using 5-F diagnostic catheter was performed to make sure that each kidney was fed by a single renal artery and the animal had normal renal vasculature. Coaxial 2.7-F microcatheter was advanced to the distal main renal artery. Under fluoroscopic guidance, 2 mL of Ankaferd mixed with 2 mL of nonionic iodinated contrast agent was slowly injected. Fluoroscopy was used to observe the deceleration of flow and stagnation. Control renal angiograms were performed just after embolization. After the procedure, the animals were observed for 1 day and then sacrificed with intravenous sodium thiopental.

Results

The technical success was observed in seven of the seven animals.. After embolization procedure, none of the animals died or experienced a major systemic adverse event. On macroscopic examination of the embolized kidneys, thrombus at the level of main renal artery formed after Ankaferd embolization was more compact compared with the thrombi that was not Ankaferd-associated, which was observed elsewhere. Microscopically, majority of the renal tubular cells (80–90 %) were necrotic, and there was epithelial cell damage in a small portion of the cells (10–20 %).

Conclusions

RAE was safe and effective in the short-term with Ankaferd in studied animals. Further studies should be conducted to better delineate the embolizing potential of this novel hemostatic agent.

Keywords

Ankaferd Embolization Renal artery Transcatheter 

Notes

Acknowledgment

The authors thank Genc Medical for financial support for the conduction of the study. Dr. A. Gaipov received grant support from the ERA-EDTA fellowship program.

Conflict of interest

All of the authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE) 2012

Authors and Affiliations

  • Orhan Ozbek
    • 1
    Email author
  • Kadir Acar
    • 2
  • Osman Koc
    • 1
  • Kadir Saritas
    • 3
  • Hatice Toy
    • 4
  • Yalcin Solak
    • 5
  • Seda Ozbek
    • 6
  • Ahmet Kucukapan
    • 1
  • Ibrahim Guler
    • 1
  • Abduzhappar Gaipov
    • 5
  • Suleyman Turk
    • 5
  • Ibrahim Celaleddin Haznedaroglu
    • 7
  1. 1.Department of Radiology, Meram School of MedicineSelcuk UniversityMeram, KonyaTurkey
  2. 2.Division of Hematology, Department of Internal Medicine, Meram School of MedicineSelcuk UniversityMeram, KonyaTurkey
  3. 3.Department of General Surgery, Veterinary FacultyAfyon Kocatepe UniversityAfyonkarahisarTurkey
  4. 4.Department of Pathology, Meram School of MedicineSelcuk UniversityMeram, KonyaTurkey
  5. 5.Division of Nephrology, Department of Internal Medicine, Meram School of MedicineSelcuk UniversityMeram, KonyaTurkey
  6. 6.Department of Radiology, Selcuklu School of MedicineSelcuk UniversitySelcuklu, KonyaTurkey
  7. 7.Division of Hematology, Department of Internal Medicine, Faculty of MedicineHacettepe UniversitySihhiye, AnkaraTurkey

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