Effect of Amnion-Derived Cellular Cytokine Solution on Healing of Experimental Partial-Thickness Burns
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Amnion-derived multipotent progenitor (AMP) cells, unlike most stem cells, have been demonstrated to be nontumorigenic and nonimmunogenic. Amnion-derived cellular cytokine solution (ACCS), a secreted product of AMP cells, is a cocktail of cytokines existing at physiological levels and has been used to accelerate epithelialization of experimental partial-thickness burns.
Using modifications of Zawacki’s guinea pig partial-thickness scald burn model, a total of 65 animals were treated with ACCS, ACCS + AMP cells, unconditioned medium (UCM) + AMP cells, or either UCM alone or saline as controls. Dosage times ranged from every other day to once a week. Percent epithelialization was serially determined from acetate wound tracings. Histology was performed on wound biopsies.
ACCS, UCM + AMP cells, and ACCS + AMP cells improved epithelialization compared with the two control groups (P < 0.05). When ACCS was delivered more frequently, statistically significant more rapid epithelialization occurred (P < 0.05). By day 7, all groups treated with ACCS had reached at least 90% epithelialization, whereas control groups were only 20–40% epithelialized (P < 0.05). Histology showed excellent regeneration of the epidermis with rete ridge formation. Hair growth occurred in ACCS-treated animals but not in the control group.
Amnion-derived cellular cytokine solution accelerates the healing of experimental partial-thickness burns. Based on these findings, a multicenter clinical trial is underway.
KeywordsHair Growth Hair Cycle BMDP Statistical Software Animal Component Skin Graft Donor Site
This material is the result of work supported with resources and the use of facilities at the Bay Pines VA Healthcare System, Bay Pines, Florida. The authors acknowledge the work of Deepak N. Naidu, MD and R. Emerick Salas, MD, who served as postdoctoral research fellows and participated in the conduct of the two experiments, providing surgical care and treatment to the animals. The work was supported in part by a grant from the Department of Defense (USAMRAA 06153010).
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