Proanthocyanidin Protects Intestine and Remote Organs Against Mesenteric Ischemia/Reperfusion Injury
- 173 Downloads
Intestinal ischemia/reperfusion (IR) induces a systemic inflammatory response and releases harmful substances that may affect the function and integrity of distant organs such as lung, liver, and kidney. We conducted this study to find out if proanthocyanidins (PA) has protective effects against mesenteric IR injury and mesenteric IR-induced intestinal and distant organ injury.
Materials and methods
Thirty-two Sprague-Dawley rats were divided into four groups: control, control + PA, IR, IR + PA. The IR and IR + PA groups were subjected to mesenteric arterial ischemia for 60 min and reperfusion for 6 h. The Control + PA and IR + PA groups were administered PA (100 mg/kg/day via oral gavage) for 7 days prior to injury insult. We collected ileal and distant organ tissues, such as pulmonary, hepatic, and kidney specimens to measure tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and nitrite plus nitrate (NOx), and we then evaluated histological changes.
In the IR group, significant increases in MDA and NOx levels and significant increases in SOD and GPx activities of intestine, liver, kidney, and lung were observed. The MDA and NOx levels were significantly lower, as were the SOD and GPx activities in the IR + PA group than that in the IR group. Although the intestine and distant organs damage scores were significantly higher in the IR group, these injuries were prevented by PA in the IR + PA group.
This study demonstrates that PA has a significant effect in the protection of the intestine and the remote organs against mesenteric IR injury.
KeywordsDistant Organ Mesenteric Ischemia Grape Seed Grape Seed Extract Decrease Lipid Peroxidation
- 12.Horton JW, White DJ (1993) Lipid peroxidation contributes to cardiac deficits after ischemia and reperfusion of the small bowel. Am J Physiol 264:H192–H1686Google Scholar
- 42.Tunc T, Uysal B, Atabek C et al (2008) Erdosteine and ebselen as useful agents in intestinal ischemia/reperfusion injury. J Surg Res. doi: 10.1016/j.jss.2008.06.017