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World Journal of Surgery

, 33:630 | Cite as

Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas

  • Sanjeet G. Patel
  • Guisheng Zhou
  • Shi-He Liu
  • Min Li
  • Jae-Wook Jeong
  • Francesco J. DeMayo
  • Marie-Claude Gingras
  • Richard A. Gibbs
  • William E. Fisher
  • F. Charles BrunicardiEmail author
Article

Abstract

Background

We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes.

Methods

To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5 −/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes.

Results

A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5 −/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence.

Conclusions

A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance.

Keywords

ANG1 Expression Gene Expression Alteration Islet Neogenesis Islet Hyperplasia Islet Cell Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This project was supported by the National Institutes of Health (NIH) grant NIDDK R01-DK46441, the Elkins Pancreas Center at Baylor College of Medicine, and the generosity of Mr. and Mrs. Walter Hecht. Gratitude is extended to the people working in Dr. Brunicardi’s laboratory for their support and technical assistance. Gratitude is also extended to Katie Elsbury for her editorial assistance and Priscilla Massey for her administrative assistance throughout this project.

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Copyright information

© Société Internationale de Chirurgie 2009

Authors and Affiliations

  • Sanjeet G. Patel
    • 1
  • Guisheng Zhou
    • 1
  • Shi-He Liu
    • 1
  • Min Li
    • 1
  • Jae-Wook Jeong
    • 2
  • Francesco J. DeMayo
    • 2
  • Marie-Claude Gingras
    • 3
  • Richard A. Gibbs
    • 3
  • William E. Fisher
    • 1
  • F. Charles Brunicardi
    • 1
    Email author
  1. 1.The Michael E. DeBakey Department of SurgeryBaylor College of MedicineHoustonUSA
  2. 2.Department of Molecular and Cellular BiologyBaylor College of MedicineHoustonUSA
  3. 3.Human Genome Sequencing CenterBaylor College of MedicineHoustonUSA

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