World Journal of Surgery

, Volume 31, Issue 4, pp 808–816 | Cite as

Platelets Promote Liver Regeneration in Early Period after Hepatectomy in Mice

  • Soichiro Murata
  • Nobuhiro Ohkohchi
  • Ryota Matsuo
  • Osamu Ikeda
  • Andriy Myronovych
  • Reiko Hoshi
Article
First Online:

Abstract

Background

Platelets contain several growth factors, including platelet-derived growth factor and hepatocyte growth factor.

Materials and methods

We examined the effects of platelet increment on liver regeneration after 70% hepatectomy. Hepatectomies were carried out in male BALB/c mice, and subsequently divided into three groups: (i) untreated mice, (ii) thrombocytotic mice induced with thrombopoietin, and (iii) thrombocytopenic mice induced with anti-platelet antibody. Growth kinetics in the liver were analyzed as a function of the liver/body weight ratio, the mitotic index, the proliferating cell nuclear antigen labeling index and Ki-67 labeling index. Activation of signal transduction pathways relating to cell proliferation were examined, including the STAT3, Akt, and ERK1/2 pathways. Platelet accumulation in the residual liver was quantified by immunohistochemistry and transmission electron microscopy.

Results

In thrombocytotic and thrombocytopenic mice, liver/body weight ratios and Ki-67 labeling indices were significantly increased and significantly decreased, respectively, compared with untreated mice 48 hours post-hepatectomy. The Akt pathway was strongly activated, and platelet accumulation was significantly increased in thrombocytotic group 5 minutes post-hepatectomy compared with normal and thrombocytopenic groups. After hepatectomy platelets accumulated in the sinusoids of liver and promoted hepatocyte proliferation in early period after hepatectomy.

Conclusion

By increasing or decreasing the platelet, marked changes in liver regeneration can occur, due to differences in cellular signaling and mitosis.

Keywords

Hepatocyte Growth Factor Proliferate Cell Nuclear Antigen Liver Regeneration Sinusoidal Endothelial Cell Hepatocyte Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgements

The authors thank Dr. Kojima and Dr. Nagasawa, University of Tsukuba, Advanced Biomedical Applications, for providing the rat anti-mouse monoclonal antibody Pm-1. We also thank Kirin Brewery Co, Takasaki, Japan, for providing the PEG-rHuMGDF, and Professor M.Yamamoto, Center for Tsukuba Advanced Research Alliance, University of Tsukuba for helpful comments and suggestions. We also thank Dr. Jon Maher, Center for Tsukuba Advanced Research Alliance, University of Tsukuba for correcting English grammar.

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Copyright information

© Société Internationale de Chirurgie 2007

Authors and Affiliations

  • Soichiro Murata
    • 1
  • Nobuhiro Ohkohchi
    • 1
  • Ryota Matsuo
    • 1
  • Osamu Ikeda
    • 1
  • Andriy Myronovych
    • 1
  • Reiko Hoshi
    • 1
  1. 1.Department of Surgery, Advanced Biomedical ApplicationsGraduate School of Comprehensive Human Sciences, University of TsukubaTsukubaJapan

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