World Journal of Surgery

, Volume 31, Issue 4, pp 654–663 | Cite as

In Vitro Models of Angiogenesis

  • Areck A. Ucuzian
  • Howard P. Greisler


Neovascularization can be categorized into two general processes: vasculogenesis and angiogenesis. Angiogenesis is the formation of new capillaries from pre-existing vessels, requiring growth factor driven recruitment, migration, proliferation, and differentiation of endothelial cells (ECs). Complex cell–cell and cell-extracellular matrix (ECM) interactions contribute to this process, leading finally to a network of tube-like formations of endothelial cells supported by surrounding mural cells. The study of angiogenesis has broad clinical implications in the fields of peripheral and coronary vascular disease, oncology, hematology, wound healing, dermatology, and ophthalmology, among others. As such, novel, clinically relevant models of angiogenesis in vitro are crucial to the understanding of angiogenic processes. We highlight some of the advances made in the development of these models, and discuss the importance of incorporating the three-dimensional cell-matrix and EC–mural cell interactions into these in vitro assays of angiogenesis. This review also discusses our own 3-D angiogenesis assay and some of the in vitro results from our lab as they relate to therapeutic neovascularization and tissue engineering of vascular grafts.


Vascular Endothelial Growth Factor Fibroblast Growth Factor Mural Cell Angiogenesis Model Growth Factor Delivery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by grants (to H.P.G.) from the National Institutes of Health (NIH R01-HL41272) and the Department of Veteran’s Affairs.


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Copyright information

© Société Internationale de Chirurgie 2007

Authors and Affiliations

  1. 1.Department of SurgeryLoyola University Medical CenterMaywoodUSA
  2. 2.Department of Cell Biology, Neurobiology, and AnatomyLoyola University Medical CenterMaywoodUSA
  3. 3.Department of Surgery and Research ServiceHines V.A. HospitalHinesUSA

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