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World Journal of Surgery

, Volume 26, Issue 4, pp 401–408 | Cite as

Recurrent Inguinal Hernia: Disease of the Collagen Matrix?

  • Hong Zheng
  • Zhongyi Si
  • Reiner Kasperk
  • Rhanjit S. Bhardwaj
  • Volker Schumpelick
  • Uwe Klinge
  • Bernd Klosterhalfen

The aim of this study was to investigate the collagen matrix in recurrent inguinal hernias. Total ribonucleic acid (RNA) was extracted from skin fibroblasts of three groups (control group I = healthy skin; control group II = plain skin scar; recurrent inguinal hernia group = skin of recurrent inguinal hernias; each n = 5). Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis were used to investigate the expression of procollagen type I/- III, MMP-1, and MMP-13 mRNAs. Both ratios of procollagen types I to III mRNAs and collagen types I to III were apparently decreased in the recurrent hernia group compared to those of both control groups (p <0.01). Significant differences were caused by the increase of both procollagen type III mRNA and collagen type III protein synthesis. A concomitant increase of MMP-1 and MMP-13 mRNAs and proteins was also observed in the recurrent hernia group and showed significant differences compared to those of both control groups I and II, respectively (p <0.01). In conclusion, the decreased ratio of collagen types I to III seems not only to be the result of a relative increase in the levels of type III procollagen mRNA but also may be the result of an increase of MMP-1 and MMP-13. The data of the present study strongly suggest recurrent inguinal hernias to be a disease of the collagen matrix and result in a clearer understanding of the underlying pathophysiology and may support specific therapeutic strategies in hernia surgery (e.g., surgical meshes).

Keywords

Northern Blot Collagen Type Northern Blot Analysis Skin Fibroblast Ribonucleic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Société Internationale de Chirurgie 2002

Authors and Affiliations

  • Hong Zheng
    • 1
  • Zhongyi Si
    • 1
  • Reiner Kasperk
    • 1
  • Rhanjit S. Bhardwaj
    • 2
  • Volker Schumpelick
    • 1
  • Uwe Klinge
    • 1
  • Bernd Klosterhalfen
    • 2
  1. 1.Department of Surgery, Technical University of Aachen, Pauwelstrasse 30, 52057 Aachen, GermanyGERMANY
  2. 2.Institute of Pathology, Technical University of Aachen, Pauwelstrasse 30, 52057 Aachen, GermanyGERMANY

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