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Aesthetic Plastic Surgery

, Volume 44, Issue 1, pp 177–185 | Cite as

Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

  • Baris CinEmail author
  • Nesibe Sinem Ciloglu
  • Shahruk Omar
  • Neslihan Kaya Terzi
Original Article Basic Science/Experimental
  • 76 Downloads

Abstract

Background

Autologous fat grafting is a common procedure performed for cosmetic and reconstructive purposes. Unpredictable graft survival is a major drawback, and a variety of improvements on technique such as using antioxidants have been reported to increase retention. The authors examined whether a natural rosemary plant extract known with antioxidant properties, rosmarinic acid, could improve the survival of the adipose tissue.

Methods

Inguinal fat pads were harvested from 24 Sprague Dawley rats and implanted to the parascapular area. Rats were divided into three groups: rosmarinic acid and ethanol as solvent (RA), ethanol (E), and serum physiologic (C). These substances were administered intraperitoneally daily for 1 week and once a week for 7 weeks. Volume and weight measurements, blood specimens, weight and volume records, and histopathologic examinations were performed and analyzed.

Results

The RA group demonstrated lower TOS, TGF-beta, TNF-alpha, and MDA values compared to E and C groups. TGF-beta increase was statistically insignificant, but TOS, TNF-alpha, MDA decrease was found statistically significant. Weight and volume losses were lower in the RA and E groups compared to the C group. The difference between the RA and E groups in terms of weight and volume loss was statistically insignificant. Histopathologically fat necrosis, inflammation, and fibrosis were less in the RA group compared to the E and C groups (p < 0.05).

Conclusion

Rosmarinic acid increased the fat graft volume retained and decreased cyst formation and abscess formation in the rat model. Further studies can be undertaken to investigate rosmarinic acid’s local application as a tumescent and safety in humans.

No Level Assigned

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Keywords

Rosmarinic acid Fat graft Rat Ethanol Alcohol Graft survival Adipose 

Notes

Funding

All of the expenses of this study have been funded by the corresponding author.

Compliance with Ethical Standards

Conflict of interest

All of the authors declare that they have no conflict of interest.

Ethical Approval

This study was approved by the ethical review board of Acibadem University. All applicable international, national, and institutional guidelines for the care and use of animals were followed.

References

  1. 1.
    Mazzola RF, Mazzola IC (2015) History of fat grafting: from ram fat to stem cells. Clin Plast Surg 42(2):147–153.  https://doi.org/10.1016/j.cps.2014.12.002 CrossRefPubMedGoogle Scholar
  2. 2.
    Coleman SR (1998) Structural fat grafting. Aesthet Surg J. 18(5):386–388.  https://doi.org/10.1016/s1090-820x(98)70098-6 CrossRefPubMedGoogle Scholar
  3. 3.
    Klein JA (1996) Tumescent technique for local anesthesia. West J Med 164(6):517PubMedPubMedCentralGoogle Scholar
  4. 4.
    Rohrich RJ, Sorokin ES, Brown SA (2004) In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg 113(1):391–395.  https://doi.org/10.1097/01.prs.0000097293.56504.00(discussion 396–397) CrossRefPubMedGoogle Scholar
  5. 5.
    Modarressi A (2013) Platlet rich plasma (PRP) improves fat grafting outcomes. World J Plast Surg 2(1):6–13PubMedPubMedCentralGoogle Scholar
  6. 6.
    Centeno RF (2006) Combination volume rejuvenation therapy of the face: fat, fillers, and Botox. Aesthet Surg J 26(4):460–464.  https://doi.org/10.1016/j.asj.2006.06.009 CrossRefPubMedGoogle Scholar
  7. 7.
    Khouri RK, Eisenmann-Klein M, Cardoso E et al (2012) Brava and autologous fat transfer is a safe and effective breast augmentation alternative: results of a 6-year, 81-patient, prospective multicenter study. Plast Reconstr Surg 129(5):1173–1187CrossRefGoogle Scholar
  8. 8.
    Cai W, Yu LD, Tang X et al (2018) The stromal vascular fraction improves maintenance of the fat graft volume: a systematic review. Ann Plast Surg 81(3):367–371.  https://doi.org/10.1097/SAP.0000000000001589 CrossRefPubMedGoogle Scholar
  9. 9.
    Phipps KD, Gebremeskel S, Gillis J et al (2015) Alternatively activated M2 macrophages improve autologous fat graft survival in a mouse model through induction of angiogenesis. Plast Reconstr Surg 135(1):140–149.  https://doi.org/10.1097/PRS.0000000000000793 CrossRefPubMedGoogle Scholar
  10. 10.
    Medina MA 3rd, Nguyen JT, Kirkham JC et al (2011) Polymer therapy: a novel treatment to improve fat graft viability. Plast Reconstr Surg 127(6):2270–2282.  https://doi.org/10.1097/PRS.0b013e3182139fc1 CrossRefPubMedGoogle Scholar
  11. 11.
    Gillis J, Gebremeskel S, Phipps KD et al (2015) Effect of N-acetylcysteine on adipose-derived stem cell and autologous fat graft survival in a mouse model. Plast Reconstr Surg 136(2):179e–188e.  https://doi.org/10.1097/PRS.0000000000001443 CrossRefPubMedGoogle Scholar
  12. 12.
    Temiz G, Sirinoglu H, Yesiloglu N et al (2016) Effects of deferoxamine on fat graft survival. Facial Plast Surg 32(4):438–443.  https://doi.org/10.1055/s-0036-1584236 CrossRefPubMedGoogle Scholar
  13. 13.
    Medina M, Lee J, Kirkham J et al (2011) Fat grafting, ischemia repurfusion, apoptosis, antioxidants. J Surg Res 165(2):323CrossRefGoogle Scholar
  14. 14.
    Hamed S, Egozi D, Kruchevsky D et al (2010) Erythropoietin improves the survival of fat tissue after its transplantation in nude mice. PLoS ONE 5(11):e13986CrossRefGoogle Scholar
  15. 15.
    Park B, Kong JS, Kang S et al (2011) The effect of epidermal growth factor on autogenous fat graft. Aesthet Plast Surg 35(5):738–744.  https://doi.org/10.1007/s00266-011-9679-y CrossRefGoogle Scholar
  16. 16.
    Alagawany M, El-Hack MEA, Farag MR et al (2017) Rosmarinic acid: modes of action, medicinal values and health benefits. Anim Health Res Rev 18(2):167–176CrossRefGoogle Scholar
  17. 17.
    Luan H, Kan Z, Xu Y et al (2013) Rosmarinic acid protects against experimental diabetes with cerebral ischemia: relation to inflammation response. J Neuroinflamm 17(10):28.  https://doi.org/10.1186/1742-2094-10-28 CrossRefGoogle Scholar
  18. 18.
    Zhang X, Ma ZG, Yuan YP et al (2018) Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKalpha/Smad3 signaling. Cell Death Dis 9(2):102.  https://doi.org/10.1038/s41419-017-0123-3 CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Jordán MJ, Lax V, Rota MC et al (2012) Relevance of carnosic acid, carnosol, and rosmarinic acid concentrations in the in vitro antioxidant and antimicrobial activities of Rosmarinus officinalis (L.) methanolic extracts. J Agric Food Chem 60(38):9603–9608CrossRefGoogle Scholar
  20. 20.
    Osakabe N, Yasuda A, Natsume M et al (2004) Rosmarinic acid inhibits epidermal inflammatory responses: anticarcinogenic effect of Perilla frutescens extract in the murine two-stage skin model. Carcinogenesis 25(4):549–557.  https://doi.org/10.1093/carcin/bgh034 CrossRefPubMedGoogle Scholar
  21. 21.
    Durling NE, Catchpole OJ, Grey JB et al (2007) Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures. Food Chem 101(4):1417–1424.  https://doi.org/10.1016/j.foodchem.2006.03.050 CrossRefGoogle Scholar
  22. 22.
    Ritschel W, Starzacher A, Sabouni A et al (1989) Percutaneous absorption of rosmarinic acid in the rat. Methods Find Exp Clin Pharmacol 11(5):345–352PubMedGoogle Scholar
  23. 23.
    Fonteles AA, de Souza CM, de Sousa Neves JC et al (2016) Rosmarinic acid prevents against memory deficits in ischemic mice. Behav Brain Res 297:91–103CrossRefGoogle Scholar
  24. 24.
    von Heimburg D, Lemperle G, Dippe B et al (1994) Free transplantation of fat autografts expanded by tissue expanders in rats. Br J Plast Surg 47(7):470–476CrossRefGoogle Scholar
  25. 25.
    Zhang Y, Chen X, Yang L et al (2015) Effects of rosmarinic acid on liver and kidney antioxidant enzymes, lipid peroxidation and tissue ultrastructure in aging mice. Food Funct 6(3):927–931.  https://doi.org/10.1039/c4fo01051e CrossRefPubMedGoogle Scholar
  26. 26.
    Wang T, Takikawa Y, Satoh T et al (2011) Carnosic acid prevents obesity and hepatic steatosis in ob/ob mice. Hepatol Res 41(1):87–92.  https://doi.org/10.1111/j.1872-034X.2010.00747.x CrossRefPubMedGoogle Scholar
  27. 27.
    Xie Y, Zheng D, Li Q et al (2010) The effect of centrifugation on viability of fat grafts: an evaluation with the glucose transport test. J Plast Reconstr Aesthet Surg 63(3):482–487CrossRefGoogle Scholar
  28. 28.
    Lin SY, Wang YY, Chen WY et al (2017) Hepatoprotective activities of rosmarinic acid against extrahepatic cholestasis in rats. Food Chem Toxicol 108(Pt A):214–223.  https://doi.org/10.1016/j.fct.2017.08.005 CrossRefPubMedGoogle Scholar
  29. 29.
    Sommer B, Sattler G (2000) Current concepts of fat graft survival: histology of aspirated adipose tissue and review of the literature. Dermatol Surg 26(12):1159–1166CrossRefGoogle Scholar
  30. 30.
    Tansey J, Sztalryd C, Hlavin E et al (2004) The central role of perilipin a in lipid metabolism and adipocyte lipolysis. IUBMB Life 56(7):379–385CrossRefGoogle Scholar
  31. 31.
    Nakamura Y, Ohto Y, Murakami A et al (1998) Superoxide scavenging activity of rosmarinic acid from Perilla frutescens Britton var. acuta f. viridis. J Agric Food Chem 46(11):4545–4550CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  1. 1.Department of Plastic, Reconstructive, and Aesthetic SurgeryHaydarpasa Numune Research and Training HospitalIstanbulTurkey
  2. 2.Department of PathologySultan Abdulhamit Han Research and Training HospitalIstanbulTurkey
  3. 3.Edirne Sultan 1. Murat Devlet Hastanesi Plastik, Rekonstrüktif ve Estetik Cerrahi KliniğiEdirne Merkez, EdirneTurkey

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