Aesthetic Plastic Surgery

, Volume 44, Issue 1, pp 177–185 | Cite as

Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

  • Baris CinEmail author
  • Nesibe Sinem Ciloglu
  • Shahruk Omar
  • Neslihan Kaya Terzi
Original Article Basic Science/Experimental



Autologous fat grafting is a common procedure performed for cosmetic and reconstructive purposes. Unpredictable graft survival is a major drawback, and a variety of improvements on technique such as using antioxidants have been reported to increase retention. The authors examined whether a natural rosemary plant extract known with antioxidant properties, rosmarinic acid, could improve the survival of the adipose tissue.


Inguinal fat pads were harvested from 24 Sprague Dawley rats and implanted to the parascapular area. Rats were divided into three groups: rosmarinic acid and ethanol as solvent (RA), ethanol (E), and serum physiologic (C). These substances were administered intraperitoneally daily for 1 week and once a week for 7 weeks. Volume and weight measurements, blood specimens, weight and volume records, and histopathologic examinations were performed and analyzed.


The RA group demonstrated lower TOS, TGF-beta, TNF-alpha, and MDA values compared to E and C groups. TGF-beta increase was statistically insignificant, but TOS, TNF-alpha, MDA decrease was found statistically significant. Weight and volume losses were lower in the RA and E groups compared to the C group. The difference between the RA and E groups in terms of weight and volume loss was statistically insignificant. Histopathologically fat necrosis, inflammation, and fibrosis were less in the RA group compared to the E and C groups (p < 0.05).


Rosmarinic acid increased the fat graft volume retained and decreased cyst formation and abscess formation in the rat model. Further studies can be undertaken to investigate rosmarinic acid’s local application as a tumescent and safety in humans.

No Level Assigned

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Rosmarinic acid Fat graft Rat Ethanol Alcohol Graft survival Adipose 



All of the expenses of this study have been funded by the corresponding author.

Compliance with Ethical Standards

Conflict of interest

All of the authors declare that they have no conflict of interest.

Ethical Approval

This study was approved by the ethical review board of Acibadem University. All applicable international, national, and institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2019

Authors and Affiliations

  1. 1.Department of Plastic, Reconstructive, and Aesthetic SurgeryHaydarpasa Numune Research and Training HospitalIstanbulTurkey
  2. 2.Department of PathologySultan Abdulhamit Han Research and Training HospitalIstanbulTurkey
  3. 3.Edirne Sultan 1. Murat Devlet Hastanesi Plastik, Rekonstrüktif ve Estetik Cerrahi KliniğiEdirne Merkez, EdirneTurkey

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