A Reliable Stem Cell Carrier: An Experimental Study in Wistar Rats
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Treatments based on cell biology need reliable and precise carriers for reaching the desired targets. For that reason, a PDO-based cell carrier was idealized, with the purpose of carrying stem cells to distant sites at room temperature.
Materials and Methods
Three modalities of the same carrier were evaluated: one containing undifferentiated human dental pulp stem cells (DPSCs); one loaded with stem cells induced to neurogenic differentiation (DPSCNs); and one without cells (Blank). The carriers were implanted in sciatic nerve gaps in 48 Wistar rats that were divided in three groups. Two other rats were included in a SHAM control group. Immunohistochemical, histological and clinical analyses were performed in two, four, six and eight weeks of time.
Efficacy of human stem cell transportation at room temperature to rats was attested. Moreover, it was possible to confirm that those cells show tropism for inflamed environments and are also prone to induction of neurogenesis in the first two weeks, vanishing after that period.
Clinical evaluation of the animals’ gait recovery shows a promising perspective of success with the inclusion of stem cell-loaded PDO tubes in nerve gaps, which may be positively compared to previously published studies.
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KeywordsStem cells PDO carrier Nerve regeneration Nerve guide Tropism for inflammation
The authors are grateful to Engineer Erich Odermatt for the materialization of the idea about the carrier; to Aesculap/B. Braun for the production of the carrier, its supply and the suture threads; to pharmacist-biochemist Cristina Tanikawa for supplying the two SHAM animals; to Laboratório Pathos Daignósticos Médicos for performing the immunohistochemical analyses; to William Adalberto Silva, biologist, and Waldemir Benedito Costa and Miguel Luís Cândido, animal laboratory technicians. They gave all the support needed for the success in the animal research.
Acquisition of the rats and biological products (such as antibodies, antibiotics and analgesics) used in the study was funded by the senior author.
Compliance with Ethical Standards
The main author has an Intellectual Property (Patent) of a biological carrier since 2008, in the USA. In August 2010, he signed a Collaboration Agreement with Aesculap/B. Braun in a potential production of the device. That is all to disclose.
All applicable institutional and national guidelines for the care and use of animals were followed.
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