Effects of Cigarette Smoke on Fat Graft Survival in an Experimental Rat Model
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A fat graft is the closest thing to being the ideal soft tissue filler. Although it has many advantages, reliability of late-term survival is a never-ending debate. Although there are observational studies that research the effect of cigarette smoke on fat graft take in clinical setting, there has not been an objective experimental animal study on the affect of smoking on fat graft survival. The aim of our study is to search if smoking has an affect on fat grafts.
Materials and Methods
Twenty-two Sprague-Dawley type rats were used. Exposure was maintained via a passive smoke exposure system. Rats were divided into three groups regarding their exposure period. At the end of the study, transferred fat grafts were extracted and weighed with a precision scale, an arterial blood sample was taken for biochemical analysis, and grafts were sent to the pathology laboratory for immunohistochemical assessment.
There were meaningful differences between the control group and the other two groups in graft weight loss, serum cotinine, tissue MDA, adipose tissue/fibrosis ratio, stem cell counts, perilipin positive cell density and inflammation density. Furthermore, we detected meaningful correlations between serum cotinine, tissue MDA and graft weight loss.
Fat graft takes with the same mechanisms as a wound heals. So like wound healing, cigarette smoke has a negative affect on fat graft survival. A fat graft is by its nature an elective procedure so to improve our late-term success, cigarette smoke exposure should be kept to a minimum for increased reliability.
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KeywordsFat grafting Smoking Tobacco Cigarette Fat graft survival Fat graft reliability
Funding from Istanbul University Scientific Research Projects Section has been granted for this project. (Project ID 23124)
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
Approval of the Istanbul University Experimental Animal Research Ethics Committee had been taken for conducting this experimental study.
For this type of study, informed consent is not required.
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