Aesthetic Plastic Surgery

, Volume 42, Issue 2, pp 343–351 | Cite as

Nanofat Increases Dermis Thickness and Neovascularization in Photoaged Nude Mouse Skin

  • Peng Xu
  • Qian Yu
  • Huizhen Huang
  • Wen Jie ZhangEmail author
  • Wei LiEmail author
Original Article Basic Science/Experimental



To investigate the effects of nanofat injection into photoaged nude mouse skin on dermis thickness, neovascularization, and cell proliferation.


Adipose-derived stem cells (ADSCs) and nanofat were prepared from human liposuction aspirates. The photoaged skin model was created using ultraviolet B (UVB) radiation onto BALB/c nude mice. A total of 24 mice were used in this study; 6 mice without treatment (natural aging) served as controls, while 18 mice were irradiated under the UVB lamp and treated with PBS (200 μl per injection area), ADSCs (1 × 106/200 μl ADSCs per injection area), or nanofat (200 μl per injection area) on the dorsal skin. Four weeks after injection, skin specimens were collected. The skin texture of each group was evaluated by general observation. Histological analyses were performed to analyze skin structure, dermis thickness, collagen fiber arrangement, capillary density, and cell proliferation.


Four weeks after injection, no obvious differences were observed between the PBS group, ADSCs group, and nanofat group by skin gross observation. From the histological analyses, the ADSCs group and the nanofat group showed obviously thicker dermis than the PBS group (P < 0.05). More capillaries were observed in skin using anti-CD31 staining in the ADSCs and Nanofat groups than was observed in the PBS group (P < 0.05). No significant differences in the average dermis proliferation index were observed between groups by anti-Ki-67 staining. However, an increased epidermal proliferation index was observed in the ADSCs and Nanofat groups, compared to that in the PBS group (P < 0.05).


Nanofat increased dermis thickness and neovascularization in photoaged skin.

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Aging Dermis Rejuvenation Stem cells 



This work was supported by the Shanghai Science and Technology Commission (No. 16ZR1419800).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

The welfare of animals used for research was respected. All applicable institutional and/or national guidelines for the care and use of animals were followed.


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© Springer Science+Business Media, LLC, part of Springer Nature and International Society of Aesthetic Plastic Surgery 2018

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People’s HospitalShanghai Jiao Tong UniversityShanghaiChina

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