Aesthetic Plastic Surgery

, Volume 36, Issue 5, pp 1260–1265 | Cite as

In Vitro N-Acetyl-l-Cysteine Promotes Proliferation and Suppresses Interleukin-8 Expression in Adipose-Derived Stem Cells

  • Lingyun Xiong
  • Jiaming Sun
  • Christoph Hirche
  • Jie Yang
  • Yanqing Yang
  • Yun Xia
  • Marcus Lehnhardt
  • Rongrong Wang
  • Xi Fu
Original Article Experimental/Special Topics

Abstract

Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify the effects of N-acetyl-l-cysteine (NAC) on the proliferation, death, and IL-8 production of ADSCs. In the presence or absence of NAC, ADSC proliferation was examined using a CCK-8 Kit, and cell death was evaluated by flow cytometry analysis. Subsequently, IL-8 mRNA expression was detected by reverse transcriptase-polymerase chain reaction and protein production by enzyme-linked immunoassay. Findings showed that cell proliferation in the NAC-treated group was a significant 1.53-fold greater than in the control group, that the apoptosis rate of ADSCs decreased by 55.4 % compared with the control group, and that the necrosis rate decreased by 48.8 %. Additionally, the IL-8 mRNA expression decreased to 46.2 ± 8.7 % that of the control group, and the IL-8 protein production decreased to 9.98 ± 0.57 %. The authors believe that NAC might be helpful in burn wound repair and regeneration by stimulating the proliferation of ADSCs, inhibiting cell death, and suppressing IL-8 production.

Level of Evidence II

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Keywords

ADSCs IL-8 NAC Proliferation 

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Copyright information

© Springer Science+Business Media, LLC and International Society of Aesthetic Plastic Surgery 2012

Authors and Affiliations

  • Lingyun Xiong
    • 1
  • Jiaming Sun
    • 1
  • Christoph Hirche
    • 2
  • Jie Yang
    • 1
  • Yanqing Yang
    • 1
  • Yun Xia
    • 1
  • Marcus Lehnhardt
    • 2
  • Rongrong Wang
    • 1
  • Xi Fu
    • 1
  1. 1.Department of Plastic and Reconstructive SurgeryUnion Hospital, Huazhong Science and Technique UniversityWuhanChina
  2. 2.Department of Hand, Plastic, and Reconstructive Surgery, BG-Trauma Centre LudwigshafenUniversity of HeidelbergLudwigshafenGermany

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