Behavioral Ecology and Sociobiology

, Volume 66, Issue 6, pp 865–878

Behavioral responses of territorial red squirrels to natural and experimental variation in population density

  • Ben Dantzer
  • Stan Boutin
  • Murray M. Humphries
  • Andrew G. McAdam
Original Paper

DOI: 10.1007/s00265-012-1335-2

Cite this article as:
Dantzer, B., Boutin, S., Humphries, M.M. et al. Behav Ecol Sociobiol (2012) 66: 865. doi:10.1007/s00265-012-1335-2


The relative scarcity of studies at the intersection of behavioral and population ecology is surprising given the presumed importance of behavior in density-dependent population regulation. Here we tested whether North American red squirrels (Tamiasciurus hudsonicus) adjust their behavior in response to local population density and whether they use rates of territorial vocalizations in their local neighborhood to assess density. We examined these relationships using 18 years of live trapping and 20 years of behavioral data that were collected across natural variation in local population density. To disentangle the effects of population density on behavior from those due to changes in per capita food abundance or changes in the frequency of antagonistic interactions with neighbors, we also experimentally manipulated population density with long-term food supplementation as well as perceived population density with long-term playbacks of territorial vocalizations. The frequency with which squirrels emitted territorial vocalizations was positively associated with local population density. In contrast, antagonistic physical interactions observed between squirrels and territorial intrusions were rare and the frequency of intrusions was weakly and negatively, not positively, associated with population density. Squirrels experiencing naturally and experimentally high density conditions spent less time in the nest and feeding but more time being vigilant. Similar density-dependent changes in behavior were observed in response to our manipulations of perceived population density, indicating that vocalization rates and not physical interactions or food abundance were the mechanism by which squirrels assessed and responded behaviorally to changes in local density.


Food supplementation Playbacks Population density Population regulation Territoriality 

Supplementary material

265_2012_1335_MOESM1_ESM.pdf (188 kb)
Fig. S1Pearson’s correlations between local squirrel density (squirrels/hectare) measured from 25 to 300 m away from the midden of interest and the frequency of territorial intruders, territorial vocalizations, and behavioral response variables (PC1 and PC2). We calculated local density by considering a circle with a radius ranging from 25 to 300 m around the midden of interest and counting the number of squirrels defending a territory within these areas. Values shown on the y-axis are absolute values of Pearson’s correlations. We used these data to determine that 150 m is an appropriate scale at which to measure local population density. At this scale, the Pearson correlation between local population density and three of the response variables (intruders, rattles, and PC1) is near the highest relative to the other scales. While the Pearson correlation between local population density measured at this scale and PC2 is not near the highest relative to the other scales, we are still likely to gain similar inferences measuring density at this scale compared to others because the Pearson correlation remains either weakly or strongly positive at all scales (PDF 187 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ben Dantzer
    • 1
  • Stan Boutin
    • 2
  • Murray M. Humphries
    • 3
  • Andrew G. McAdam
    • 1
    • 4
  1. 1.Department of ZoologyMichigan State UniversityEast LansingUSA
  2. 2.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  3. 3.Department of Natural Resource SciencesMcGill UniversitySte-Anne-de-BellevueCanada
  4. 4.Department of Integrative BiologyUniversity of GuelphGuelphCanada

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