Behavioral Ecology and Sociobiology

, Volume 65, Issue 2, pp 401–413

How many color metrics do we need? Evaluating how different color-scoring procedures explain carotenoid pigment content in avian bare-part and plumage ornaments

  • Michael W. Butler
  • Matthew B. Toomey
  • Kevin J. McGraw
Methods

Abstract

For a variety of technical and conceptual reasons, biologists have come to use several different methods to quantify the colors of animals. However, the relative abilities of these different color-scoring procedures to capture variation in the actual color-generating mechanisms—pigment or structural composition of the integument—have never been tested systematically. Here, we examined which commonly employed color metrics predict carotenoid content of ornaments in three avian species (house finch Carpodacus mexicanus, mallard duck Anas platyrhynchos, and zebra finch Taeniopygia guttata). We used spectrophotometry to measure reflectance spectra from beak and feather tissue, calculated numerous color metrics (e.g., hue, chroma, brightness, principal components, and tetrahedral color space position) from these spectra, and determined carotenoid content at the site of color measurement with high-performance liquid chromatography. We found that several principal component, tristimulus, and avian visual model metrics significantly correlated with carotenoid content of house finch feathers and duck beaks. Carotenoid content of mallard beaks was most closely correlated with brightness and saturation metrics, whereas in house finch feathers, carotenoid concentration was best captured by hue and saturation metrics. According to tristimulus scores and visual models, we found that the ultraviolet portion of the spectrum was not an essential predictor of variation in carotenoid content. Also, visual model chromatic contrasts generally were not significant predictors of carotenoid content, although some achromatic contrasts and tetrahedral color space vector parameters were. Our results indicate that numerous methods, especially tristimulus scores, are suitable for capturing pigment-based color variation in two carotenoid-containing ornaments, and we discuss the merits and shortcomings of these different approaches. In contrast, there were no significant relationships between any color metrics and the carotenoid content of zebra finch beaks, suggesting that other color-generating mechanisms besides carotenoids may contribute to color variability in this species.

Keywords

Anas platyrhynchos Carpodacus mexicanus Ornamentation Principal components analysis Taeniopygia guttata Tristimulus Ultraviolet Visual model 

List of symbols and abbreviations

B

Brightness

H

Hue

HPLC

High-performance liquid chromatography

LWS

Long wavelength sensitive

MWS

Medium wavelength sensitive

PC

Principal component

PCA

Principal components analysis

S

Saturation

SWS

Short wavelength sensitive

UV

Ultraviolet

UV-Vis

Ultraviolet and human-visible

UVS

Ultraviolet sensitive

VS

Violet sensitive

θ

Theta in visual model; relative stimulation of SWS, MWS, and LWS photoreceptors

φ

Psi in visual model: stimulation of the UV/V sensitive photoreceptors

r

R in visual model: chromaticity or spectral purity

Supplementary material

265_2010_1074_MOESM1_ESM.pdf (135 kb)
ESM 1(PDF 135 kb)
265_2010_1074_MOESM2_ESM.pdf (165 kb)
ESM 2(PDF 164 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Michael W. Butler
    • 1
  • Matthew B. Toomey
    • 1
  • Kevin J. McGraw
    • 1
  1. 1.Arizona State University, School of Life SciencesTempeUSA

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