Strategies of a parasite of the ant–Acacia mutualism
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Mutualisms can be exploited by parasites—species that obtain resources from a partner but provide no services. Though the stability of mutualisms in the presence of such parasites is under intensive investigation, we have little information on life history traits that allow a species to be a successful mutualist or rather a parasite, particularly in cases where both are closely related. We studied the exploitation of Acacia myrmecophytes by the ant, Pseudomyrmex gracilis, contrasting with the mutualistic ant Pseudomyrmex ferrugineus. P. gracilis showed no host-defending behavior and had a negative effect on plant growth. By preventing the mutualist from colonization, P. gracilis imposes opportunity costs on the host plant. P. gracilis produced smaller colonies with a higher proportion of alates than did the mutualist and thus showed an “r-like” strategy. This appears to be possible because P. gracilis relies less on host-derived food resources than does the mutualist, as shown by behavioral and stable isotope studies. We discuss how this system allows the identification of strategies that characterize parasites of mutualisms.
KeywordsAcacia Ant–plant interaction Mutualism Parasitism Stable isotopes
Mutualisms are interactions among members of different species that lead to net fitness benefits for all partners involved. In general, mutualisms are based on the exchange of resources and services that the individual species would obtain or produce less efficiently (Bronstein 1994). As these resources can be attractive to non-mutualists as well, mutualisms are in danger of being invaded by other species, which make use of the rewards provided by a partner without rendering the respective service. Such species are termed cheaters, exploiters, or parasites of the mutualism (Bronstein 2001; Wilkinson and Sherratt 2001; Yu 2001), and the stability of mutualisms in their presence is the subject of intensive empirical and theoretical research (Gaume and McKey 1999; Hoeksema and Bruna 2000; Bronstein 2001; Yu 2001; Kiers et al. 2003; Stanton 2003; Yu et al. 2004; Edwards et al. 2006; Ferrière et al. 2007).
Some obligate mutualisms are transmitted vertically; during reproduction, the host transmits symbiont offspring to its own offspring. Such vertical transmission is known, e.g., from scale insects or aphids living symbiotically with certain ant species, where some mutualists are actively carried by founding ant queens during the nuptial flight (Buschinger et al. 1987; Klein et al. 1992). Vertical transmission furthermore is common in endosymbiotic mutualisms among, e.g., insects and bacteria such as Wolbachia and Blochmannia (Zientz et al. 2004, 2005). Such mutualisms are considered evolutionarily stable and, in fact, are often characterized by a high level of phylogenetic congruency among partners (Sauer et al. 2000; Clark et al. 2001).
Yet, the majority of mutualisms are transmitted horizontally; the partners must establish the interaction anew in each generation. This mode of transmission characterizes several common mutualisms such as plant pollination or dispersal by animals, mycorrhiza, or bacterial nodulation of roots and applies to both obligate and facultative mutualisms. As the partners have to rejoin at least after each reproductive event, such mutualisms are easily exploited and their evolutionary stability remains an open issue (Wilkinson and Sherratt 2001). Recently, Ferrière et al. (2007) examined the conditions under which cooperation (i.e., mutualistic behavior) can be evolutionarily stable in the presence of exploiters. However, empirical information is scarce on characteristic life history traits of exploiters and on the strategies allowing the successful exploitation of host resources.
The aim of this study is to fill this gap, using ant–plant mutualists and their parasites as a model. Obligate ant plants (myrmecophytes) gain protection from their enemies by resident ants, which are provided with nesting space and, in most cases, food rewards (Bronstein 1998; Heil and McKey 2003). They are, however, also targets for exploitation, and parasites of ant–plant mutualisms have repeatedly been described. Central American swollen thorn acacias, for example, can be inhabited by the two non-defending parasitic ant species Pseudomyrmex nigropilosus (Janzen 1975) and Camponotus planatus (Raine et al. 2004). Similar cases of ants failing to defend their host have also been described for the ant plants Leonardoxa africana ssp. africana (Gaume and McKey 1999) and Cordia alliodora (Tillberg 2004), while other types of exploitation encompass ants that castrate their plant host, i.e., that actively cut off the host plant's flowers in order to re-direct resource allocation from plant reproduction to vegetative growth (Yu and Pierce 1998; Stanton et al. 1999; Izzo and Vasconcelos 2002).
The putative parasite studied here, Pseudomyrmex gracilis, is generally considered a generalist, twig-nesting ant but has been reported to live in thorns of myrmecophytic swollen thorn acacias (Skwarra 1935; Wheeler 1942; Ward 1993). The species is part of the P. gracilis group and is taxonomically closely related to P. nigropilosus (Ward and Downie 2005). The mutualist used for comparison, Pseudomyrmex ferrugineus, belongs to the P. ferrugineus group and thus forms part of a different clade of the same genus (Ward 1993). Ants of this species protect their host from herbivores and encroaching vegetation (Janzen 1966, 1974), cannot be found nesting outside of their host plants, only feed on host-derived food resources (Heil et al. 2004), and are physiologically adapted to the specific composition of extrafloral nectar secreted by their host plants (Heil et al. 2005).
Here, we test the hypothesis that P. gracilis parasitizes the ant–Acacia mutualism by making use of the host-derived resources without rendering a protective service, and we characterize the life history traits that distinguish it from P. ferrugineus. We compared the defensive behavior and the effect on host plant growth of the two ant species. As parasites of mutualisms can impose opportunity rather than direct costs (Yu and Pierce 1998), we also studied the effects of Acacia occupation by P. gracilis on colony founding and inhabitation by the mutualist. Furthermore, if P. gracilis indeed fails to protect its host plant, we should expect its nutrition and reproduction to be less dependent on its host plant than in case of P. ferrugineus. Putative adaptations to the different lifestyles therefore were investigated by comparing colony structures of the two ant species and by studying food use via behavioral observations and stable isotope analysis. As stable isotopes are transported in well-defined manners up the food chain, natural abundances of stable isotopes are widely used in studies of trophic structures. For example, 13C is usually transported without significant quantitative change across tropic levels and thus can be used to identify an organism's main food source by comparing the organism's 13C signature with that of its putative food source. 15N, in contrast, is enriched in consumers due to discrimination among the different isotopes by the respective metabolic enzymes, hence clearly marking increases in trophic level (Peterson and Fry 1987; Post 2002; Thompson et al. 2005).
The type and availability of food affects the life history traits of a species and might importantly affect the trade-off between investment in reproduction and in growth or maintenance. Accordingly, Janzen (1975) hypothesized that parasitic ants which, by not protecting their host plant, live on an ephemeral resource should invest early during colony development in the production of alates. We therefore finally studied colony sizes and alate–worker ratios.
Our predictions are: (1) P. gracilis does not defend its host plant but does make use of host-derived resources (food and nesting space), and it should use external resources more than does the mutualist, P. ferrugineus, (2) the presence of a P. gracilis colony prevents colonization by P. ferrugineus, and (3) P. gracilis should allocate more resources to reproduction at smaller colony sizes.
Materials and methods
Study sites, ant, and plant species
The study was conducted during several field stays (April–May 2004, September 2005, September 2006, and February 2007) in the state of Oaxaca, México. Most investigations were conducted on extensively used pastures approximately 15 km west of Puerto Escondido in the coastal area of Oaxaca (15° 55.596′ N and 97° 9.118′ W, elevation 15 m). Additional observations were made on a pasture on the Isthmus of Tehuantepec approximately 25 km north of Matias Romero, Oaxaca (17° 6.020′ N and 95° 55.860′, elevation 140 m). Species of myrmecophytes investigated were Acacia collinsii Safford, Acacia cornigera (L.) Willendow, Acacia hindsii Bentham, and A. chiapensis Safford (determined following Janzen 1974). Ant species considered were P. gracilis Roger and P. ferrugineus Smith (determined following Ward 1993). While the first survey was conducted for all four myrmecophytic Acacia species growing at our study sites, we focused the experimental part of our study on A. hindsii, thus controlling for the effects of host plant species.
Survey of inhabitation and state of plants
Inhabitations by the different ant species were studied at both sites in April and May 2004. We randomly selected 316 individual shrubs and scored the ant species that inhabited each plant, as defined by workers entering or leaving hollow thorns.
The effect of ant occupancy on the current state of the plant was investigated using plants that carried single colonies of one of the two ant species in September 2005. As many more plants are inhabited by P. ferrugineus than by P. gracilis, all A. hindsii plants inhabited only by P. gracilis were selected on two pastures (34 plants in total) and their state was estimated as follows: (1) the numbers of dead and living shoot tips were counted to calculate the percentage of dead tips, (2) the degree of herbivory was estimated to the nearest 10% of total leaf area missing, and (3) the level of overgrowth was estimated as the percent of total leaf area covered by other plants (ten categories, from 0 = no contact by other plants to 10 = plant completely overgrown by other plants). Then, the nearest plant inhabited only by P. ferrugineus that had a size differing by <20% to the P. gracilis-inhabited plant in question was selected, and the same parameters were quantified. Differences between plants inhabited by the two ant species were tested with Wilcoxon paired tests for each of the three parameters considered.
Effects of ant occupancy on growth rate were studied from September 2006 until February 2007 (end of rainy and mid dry season) by marking 44 A. hindsii saplings (heights 0.4–2.0 m) on one pasture. We rated the degree of dominance of ant species i, D i, on an ordinal scale as follows: 0 = no ant of the respective species present, 1 = a single ant present, 2 = few (2–10) ants present on <25% of leaves, 3 = workers on 25–50% of leaves, 4 = workers on 50–75% of leaves, 5 = entire plant occupied or patrolled. Eight plants in the dataset hosted colonies of both species. The relative degree of dominance by P. gracilis is defined as the difference between the two measures: D diff = D gracilis − D ferrugineus. The response variable, total shoot length of the plants, was measured to the nearest 5 cm. The site was then revisited in February 2007 and the total shoot length was remeasured in order to calculate relative growth rates: (final total shoot length−initial total shoot length)/(initial total shoot length).
Defensive and feeding behavior of ants
The defensive behavior of ants was categorized for 147 colonies of P. ferrugineus and 60 colonies of P. gracilis. Each of these colonies was the only colony inhabiting the respective host plant. As the strength of the recruitment when ant colonies are disturbed is largely independent of the initial activity level and because this behavior best reflects the maximum defense intensity, we challenged the colonies by touching the plant surface with forceps. The resulting ant behavior was categorized as follows: A: workers patrolling on >75% of leaves, rapid recruitment, and highly aggressive behavior; B: workers patrolling on >50% of leaves, slow recruitment, aggressive behavior; C: workers patrolling <50% of leaves, slow recruitment, mixed aggression and escape behavior; D: few workers patrolling, no recruitment, escape behavior; E: less than ten workers, escape behavior; F: no workers on plant surface.
Feeding behavior of ants was investigated as described previously (Heil et al. 2004). Eight colonies of P. gracilis and 12 colonies of P. ferrugineus were offered small items (length 1–2 mm) of dead plant material, boiled egg, insect parts, boiled ham, and food bodies of the same Acacia species but derived from another individual. All items were placed on the rachis or blade of young, food-body producing leaves (ten replicates per type of item and per shrub). These experiments were conducted during the peak ant activity between 10:00 a.m. and noon (LC, unpublished data; see also Raine et al. 2002). Ant behavior was observed until the item was (a) discarded actively from the plant, (b) accepted, i.e., carried into a thorn, or (c) ignored by 20 ants.
Natural abundance of stable isotopes
As a more integrative method to study the nutrition of ant colonies, we compared the natural abundances of stable isotopes (13C and 15N) in the ants and their respective host plants. We collected samples of adult workers, larvae, and leaves from A. hindsii plants (n = 21 for P. ferrugineus and n = 29 for P. gracilis), placed them in microporous capsules, and dried them immediately over silica gel. Samples were transported to Germany, redried until constant weight at 70°C, finely ground, and weighed into tin capsules (0.7–2 μg). Analysis of abundances of 13C and 15N was conducted using a stable isotope ratio mass spectrometer (Finnigan Delta+XL) coupled online with a carbon–hydrogen–nitrogen combustion unit (CE 1110) using a ConFlo III open split interface (Werner et al. 1999). Absolute amounts of the heavy stable isotopes are extremely low and thus cannot be reliably quantified. Quantities of stable isotopes in the samples are therefore determined as the ratio R of heavy to light isotopes (13C/12C and 15N/14N) and then compared to element-specific standards Vienna Pee Dee Belemnite (VPDB) for C and N2 in atmospheric air for N (Peterson and Fry 1987). The results are reported as abundance “δX [‰]” where δX = [(R sample / R standard) − 1] × 1,000. The anchor to the international VPDB scale was NBS22 with an assigned value of −29.78‰, the δ 15N scale was anchored using IAEA-N1 with an assigned δ 15N of +0.43‰. All measurements were calibrated against working reference materials co-combusted within the same measurement sequence (“IT principle”; Werner and Brand 2001).
Allocation to ant reproduction
Colony sizes and ratios of alates to workers were characterized for 11 colonies each of P. gracilis and P. ferrugineus. Only colonies that exclusively inhabited a single plant were chosen. Plants were 0.90–1.70-m high and had one clearly dominant main shoot and twigs of maximally the second order. Plants were cut into pieces of 20–30-cm length and put into plastic bags with ether. Bags were kept closed for at least 10 min and the contents of all thorns were then collected in 70% ethanol, transported to Germany, and counted using a binocular microscope separately for larvae (four stages separated according to size and structure), pupae, workers, and alates. Larvae of alates and workers could only be distinguished for the last three stages of larvae and pupae.
Direct interactions among ant species
Though colonies of P. ferrugineus and P. gracilis sometimes occur on the same shrub, direct interactions among workers were rarely observed. The two species usually were spatially separated (i.e., small colonies inhabiting distant twigs), and P. gracilis generally actively avoids encounters with other animals by, e.g., moving to the opposite side of the twig. Direct encounters between workers were therefore experimentally provoked by transferring single workers from an A. hindsii plant exclusively inhabited by one species to a plant exclusively inhabited by the other species. A total of 50 workers of each species were transferred among ten plants (five workers per colony) and their behavior was observed for the next 5 min or until they left the plant or were killed by the resident ants.
To further assess whether P. gracilis prevents plants from being colonized by the mutualist, we collected founding queens of P. ferrugineus that had newly entered thorns in September 2005. These queens were placed on ant-free plants, plants inhabited by P. ferrugineus, or plants inhabited by P. gracilis. We then observed for 30 min whether queens were attacked, stayed on, or left the plant.
Statistical tests were conducted using Statistical Package for the Social Sciences (SPSS) version 15.0 (SPSS Inc.) and were selected according to the respective experimental design and data structure. Non-parametric tests were applied whenever the data did not fit the assumptions made by the respective parametric tests in order to base the conclusions drawn on conservative estimates of significance.
Ant occupancy of Acacia plants and behavioral observations
At our field sites, many myrmecophytic Acacia plants hosted colonies of ant species not belonging to the P. ferrugineus group. These ants often occupied only small parts of large plants that were not visited by the resident mutualist colony. However, P. gracilis was particularly common, occupying 26% of A. hindsii plants (n = 60), 44% of A. chiapensis (n = 115), 2% of the A. cornigera (n = 89), and 4% of A. collinsii (n = 52).
Many saplings of <1-m height hosted small colonies of more than one species of Pseudomyrmex on separate branches. Overall, we observed P. ferrugineus, Pseudomyrmex peperi, P. gracilis, Pseudomyrmex mixtecus, and P. nigropilosus (determined following Ward 1993) on young plants of A. hindsii. Founding queens occurred mainly in September and October and occasionally patrolled plant surfaces at high numbers, including one observation of approximately 30 queens of four Pseudomyrmex species on one sapling of 50-cm height without obvious aggressive behavior. Larger colonies of P. ferrugineus usually dominate their host plants, but P. gracilis and other ant species from different genera can inhabit the lowest parts of the trunk and the lowest major branches of plants >4 m high. Only on shrubs 0.5–2-m tall were P. gracilis colonies ever the only inhabitant observed.
P. gracilis queens were regularly found in freshly opened thorns during September 2005 and twice were observed gnawing an entrance hole into a young thorn. Workers of P. gracilis were also observed to open new thorns, and, in fact, entrance holes on plants inhabited by P. gracilis have a shape different from holes in thorns inhabited by P. ferrugineus. Though both P. gracilis and P. ferrugineus workers leave their host plant, only P. gracilis workers were observed to carry off items from the host into thorns.
Effect of occupancy on plant condition
Defensive and feeding behavior of ants
Natural abundance of stable isotopes
Colony size and proportion of alates
Interactions among ants and colony founding
P. gracilis workers are only conditionally aggressive, while P. ferrugineus workers are always so. P. gracilis workers on their own host plants successfully attacked and killed P. ferrugineus workers (39/50 trials); but when P. gracilis workers were placed on P. ferrugineus-inhabited host plants, P. gracilis workers engaged in evasive behavior, usually avoided detection (28/50), and always successfully avoided being killed (0/50 trials). As a result, P. gracilis workers were significantly more likely to escape attack when placed on P. ferrugineus-inhabited plants (50/50 trials) than were P. ferrugineus workers placed on P. gracilis-inhabited plants (11/50 trials; Pearson chi-square χ2(df = 1) = 63.93, p < 0.001).
We studied behavioral and life history traits of a putatively parasitic and a mutualistic ant species. P. gracilis exploited host plant resources (food and nesting space provided by Acacia hosts) though it was found to depend less on host-derived food sources than the mutualist, P. ferrugineus. As being inhabited by P. gracilis negatively affected host plant state and growth rate and as this ant can exclude the mutualist from colonization, we conclude that P. gracilis is a parasite of the mutualism between Acacia myrmecophytes and ants of the P. ferrugineus group. Both species are congeneric and inhabit the same hosts, which largely rules out different environments and phylogenetic histories as the causes for their different ecological lifestyles. Instead, the reduced dependence on host-derived resources, the smaller colony sizes, and the higher allocation to alates in P. gracilis likely represent adaptations to this particular lifestyle and suggest some candidate traits that could generally characterize parasites of mutualisms.
The differences in host plant condition could be explained by the two ant species' behavior. Mutualistic P. ferrugineus ants were more active on the plant surface and more aggressive than workers of P. gracilis, which usually move to the opposite side of the leaf or twig when facing larger intruders (LC, personal observations). Items not accepted as food sources were ignored by P. gracilis rather than discarded from the plant, as regularly observed for mutualistic plant–ants (Janzen 1974; Davidson et al. 1988; Fiala et al. 1989; Fiala and Maschwitz 1990; Heil and McKey 2003). In all these traits, P. gracilis is similar to other non-protecting parasites of ant–plant mutualisms, such as P. nigropilosus (Janzen 1975), Cataulacus mckeyi (Gaume and McKey 1999), or C. planatus (Raine et al. 2004).
However, any ant species that forages for insects should—due to its mere presence—reduce herbivory on an inhabited plant as compared to a completely predator-free one (Heil and McKey 2003). It is therefore a matter of discussion whether or not a species that provides a lower—but not zero—service can be regarded as parasite of a mutualism. In fact, hosts often can be inhabited by several species of partners that differ in the quality of service they provide. Cases for which this has been described comprise, besides ant–plant mutualisms (Izzo and Vasconcelos 2002; Feldhaar et al. 2003; Bruna et al. 2004; Tillberg 2004; Frederickson 2005; Gaume et al. 2005a, b), very different types of interactions such as symbioses between plant roots and rhizobial bacteria (West et al. 2002; Denison and Kiers 2004) and between roots and mycorrhizal fungi (West et al. 2002; Sanders 2004). While Feldhaar et al. (2003) reported a “succession” of ant species on some Macaranga species but did not find a regular parasite, Raine et al. (2004) found C. planatus ants to actively exclude workers of P. ferrugineus from extrafloral nectaries of Acacia mayana and therefore defined this species as a parasite. Similarly, Ferrière et al. (2007) stated that a true parasite of the mutualism should exclude the mutualist, a phenomenon that indeed was found in our study: P. gracilis workers specifically attacked P. ferrugineus workers and queens when encountered on their plant. In summary, P. gracilis can impose severe opportunity costs on the plant by actively preventing the establishment of the protective mutualism and thus indeed can be termed a parasite of the ant–Acacia mutualism (Yu and Pierce 1998; Yu 2001; Ferrière et al. 2007).
How can P. gracilis survive on Acacia myrmecophytes that so obviously suffer from its presence (Fig. 1c)? When offered putative external food sources, P. ferrugineus ants only accept Acacia food bodies (Heil et al. 2004). In contrast, P. gracilis accepted external protein sources such as pieces of ham, egg, and dead insects almost as often as food bodies. These differences in food resource utilization are reflected in the digestive capacities of the two ant species (Heil et al. 2005) and were confirmed by natural abundances of stable isotopes. Stable isotopes have been used to study nutrient fluxes among ant plants and their ants (Sagers et al. 2000; Fischer et al. 2002), thereby for instance defining differences between mutualistic and supposedly parasitic ants (Tillberg 2004). In the present study, δ 13C and δ 15N in P. gracilis ants (workers and larvae) were significantly higher than in the respective host plants, which indicate that the body tissue of larvae and workers contained non-host material. In contrast, no such differences were found for P. ferrugineus; hence, the entire body tissue of these ants was derived from host material. The magnitudes of differences among plant and consumers as found in our study are surprisingly low, as usually the step of one trophic level is characterized by an increase in δ 15N of 3.4‰ (Post 2002; Thompson et al. 2005). Similarly, low differences in natural abundances of stable isotopes between presumed food source and ant also characterize the Cecropia–Azteca symbiosis and were interpreted as a result of nutrient fluxes from the ant back to the plant (Sagers et al. 2000). It remains to be tested whether such nutrient fluxes can also be found in the Acacia–Pseudomyrmex mutualism. However, and most importantly in the context of the present study, our results demonstrate that P. gracilis is nutritionally less dependent on its host plant than is P. ferrugineus.
Further adaptations to these different lifestyles were investigated at the level of colony size and allocation to reproduction. P. gracilis produced much smaller colonies with a higher proportion of alates than did P. ferrugineus, and alates appeared in smaller colonies of P. gracilis, relative to P. ferrugineus. Similar patterns characterize the Macaranga–Crematogaster system, where “early successional” ant species produced alates at smaller colony sizes than did “late successional” species (Feldhaar et al. 2003). Such differences in alate allocation might be an artifact of sampling outside P. ferrugineus' reproductive peak. However, observations over several years have found that the highest numbers of founding queens of both species occur in September and October (LC and MH, personal observations).
To generalize our findings to cooperation theory, we note that P. gracilis' reduced reliance on host resources reduces the correlation between host fitness and symbiont fitness (Sachs et al. 2004). As a result, P. gracilis' fitness should be less adversely affected by the loss of host plant tissue than is P. ferrugineus' fitness, and if P. gracilis gains more total fecundity by avoiding investment in patrolling workers than it loses by living in a smaller and more rapidly dying plant, non-patrolling behavior can be maintained (Edwards et al. 2006). Hence, the parasitic lifestyle in P. gracilis is to reproduce early and therefore at small colony sizes (an “r-like” strategy), giving it more opportunity to have colonized new hosts when its old host dies.
Many mutualisms consist of groups of interacting species with different degrees to specialization to the host, including exploiters. Thus, the traits documented here might also characterize other parasites of mutualisms. For example, of the strains of Rhizobia bacteria that infect plant roots, some establish a mutualistic symbiosis by starting nitrogen fixation, while other strains do not and therefore parasitize the system (Kiers et al. 2003; Denison and Kiers 2004). Host sanctions against non-fixing strains and strikingly different reproduction maintenance trade-offs led to well-defined life history characteristics that differ according to the respective strategies and that allow a co-occurrence of these different strategies (West et al. 2002; Denison and Kiers 2004). Though our study system consists of only two ant species, it thus appears likely that the results of the present study can be broadly applied, i.e., that r-like strategies in general characterize parasites of mutualists.
We are grateful to Judy Bronstein, Brigitte Fiala, and Douglas Yu for many thoughtful comments on earlier versions of this manuscript and to Douglas Yu for helping with the statistics and the English. Special thanks are to Juan Carlos Silva Bueno for field assistance and to Heike Geilmann (Berkeley Geochronology Center IsoLab Jena) for performing the routine isotope ratio analyses for this study. Financial support by the Deutsche Forschungsgemeinschaft (grant He 3169/4–2) and the Max Planck Society for the Advancement of Science is gratefully acknowledged. The experiments presented here comply with the current laws of Mexico and Germany.
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