Behavioral Ecology and Sociobiology

, Volume 53, Issue 4, pp 254–261 | Cite as

Variation in nesting patterns affecting nest temperatures in two populations of painted turtles (Chrysemys picta) with temperature-dependent sex determination

Original Article


Mechanisms maintaining sex ratios in populations with temperature-dependent sex determination (TSD) remain elusive. Although geographic variation in embryonic sex determination (i.e., pivotal temperature) has been widely investigated in reptiles exhibiting TSD, no previous studies have directly addressed geographic variation in maternal behavior affecting nest thermal conditions. I evaluated patterns of nest-site selection and its effects on thermal and hydric nest conditions for a population of painted turtles (Chrysemys picta bellii) exhibiting TSD in New Mexico. These results are compared to data collected from a well-studied, conspecific population experiencing relatively cooler climatic conditions in Illinois. Since canopy vegetation cover reduces nest temperatures in Illinois, I expected females in New Mexico to nest under high amounts of canopy vegetation cover. However, females from New Mexico placed nests under significantly less canopy vegetation cover, but closer to standing water, than did females from Illinois. Experimental nests in New Mexico demonstrated that increased canopy vegetation cover and soil moisture reduced nest temperatures. By nesting close to standing water rather than under canopy vegetation cover, females in New Mexico nested in habitats more closely associated with maximizing moisture around nests rather than reducing nest temperatures through shading. Mean July nest temperatures were similar for both populations. Since nest hydric conditions affect hatching success and hatchling size in C. picta, nesting patterns in New Mexico may primarily reflect selection for microhabitats affecting offspring survivorship or size.


Maternal effects Nesting behavior Nest-site choice 


  1. Bull JJ (1983) Evolution of sex determining mechanisms. Cummings, Menlo Park, Calif.Google Scholar
  2. Bull JJ, Vogt RC, Bulmer MG (1982a) Heritability of sex ratio in turtles with environmental sex determination. Evolution 36:333–341Google Scholar
  3. Bull JJ, Vogt RC, McCoy CJ (1982b) Sex determining temperatures in turtles: a geographic comparison. Evolution 36:326–332Google Scholar
  4. Bulmer MG, Bull JJ (1982) Models of polygenic sex determination and sex ratio control. Evolution 36:13–26Google Scholar
  5. Cagle KD, Packard GC, Miller K, Packard MJ (1993) Effects of the microclimate in natural nests on development of embryonic painted turtles, Chrysemys picta. Funct Ecol 7:653–660Google Scholar
  6. Christiansen JL, Moll EO (1973) Latitudinal reproductive variation within a single subspecies of painted turtle, Chrysemys picta bellii. Herpetologica 29:152–163Google Scholar
  7. Ernst CH, Lovich JE, Barbour RW (1994) Turtles of the United States and Canada. Smithsonian Institution Press, Washington, D.C.Google Scholar
  8. Everitt BS, Dunn G (1991) Applied multivariate data analysis. Halsted, New YorkGoogle Scholar
  9. Ewert MA (1976) Nests, nesting and aerial basking of Macroclemys under natural conditions, and comparisons with Chelydra (Testudines: Chelydridae). Herpetologica 32:150–156Google Scholar
  10. Ewert MA, Jackson DR, Nelson CE (1994) Patterns of temperature-dependent sex determination in turtles. J Exp Zool 270:3–15Google Scholar
  11. Harlow PS, Taylor JE (2000) Reproductive ecology of the jacky dragon (Amphibolurus muricatus): an agamid lizard with temperature-dependent sex determination. Aust Ecol 25:640–652CrossRefGoogle Scholar
  12. Janzen FJ (1994a) Climate change and temperature-dependent sex determination in reptiles. Proc Natl Acad Sci USA 91:7487–7490PubMedGoogle Scholar
  13. Janzen FJ (1994b) Vegetational cover predicts the sex ratio of hatchling turtles in natural nests. Ecology 75:1593–1599Google Scholar
  14. Janzen FJ, Morjan CL (2001) Repeatability of microenvironment-specific nesting behaviour in a turtle with environmental sex determination. Anim Behav 61:73–82CrossRefGoogle Scholar
  15. Janzen FJ, Paukstis GL (1991) Environmental sex determination in reptiles: ecology, evolution, and experimental design. Q Rev Biol 66:149–179PubMedGoogle Scholar
  16. Karlin S, Lessard S (1986) Theoretical studies on sex ratio evolution. Princeton University Press, Princeton N.J.Google Scholar
  17. MacCulloch RD, Secoy DM (1983) Movement in a river population of Chrysemys picta bellii in southern Saskatchewan. Can J Zool 61:1499–1509Google Scholar
  18. Morjan CL (2002) Temperature-dependent sex determination and the evolution of sex ratio in the painted turtle, Chrysemys picta. PhD dissertation, Iowa State University, Ames, IowaGoogle Scholar
  19. Morjan CL, Janzen FJ (2003) Nest temperature is not related to egg size in a turtle with temperature-dependent sex determination. Copeia (in press)Google Scholar
  20. Morjan CL, Valenzuela N (2001) Is groung-nuzzling by female turtles associated with soil surface temperatures? J Herpetol 35:668–672Google Scholar
  21. Mousseau TA, Fox CW (eds) (1998) Maternal effects as adaptations. Oxford University Press, OxfordGoogle Scholar
  22. Mrosovsky N (1988) Pivotal temperatures for loggerhead turtles (Caretta caretta) from northern and southern nesting beaches. Can J Zool 66:661–669Google Scholar
  23. National Climatic Data Center (1999) Climatological data. New Mexico, July 1999. Asheville N.C.Google Scholar
  24. National Climatic Data Center (2000a) Climatological data. New Mexico, July 2000. Asheville, N.C.Google Scholar
  25. National Climatic Data Center (2000b) Climatological data. Illinois, July 2000. Asheville, N.C.Google Scholar
  26. Packard GC, Packard MJ (2000) Developmental plasticity in painted turtles Chrysemys picta. Funct Ecol 14:474–483CrossRefGoogle Scholar
  27. Packard GC, Packard MJ (2001) Environmentally induced variation in size, energy reserves and hydration of hatchling painted turtles, Chrysemys picta. Funct Ecol 15:481–489CrossRefGoogle Scholar
  28. Packard GC, Packard MJ, Birchard GF (1989) Sexual differentiation and hatching success by painted turtles incubating in different thermal and hydric environments. Herpetologica 45:385–392Google Scholar
  29. Ratterman RJ, Ackerman RA (1989) The water exchange and hydric microclimate of painted turtle (Chrysemys picta) eggs incubating in field nests. Physiol Zool 62:1059–1079Google Scholar
  30. Ream C, Ream R (1966) The influence of sampling methods on the estimation of population structure in painted turtles. Am Midl Nat 75:325–338Google Scholar
  31. Roosenburg WM (1996) Maternal condition and nest site choice: an alternative for the maintenance of environmental sex determination? Am Zool 36:157–168Google Scholar
  32. Schwarzkopf L, Brooks RJ (1985) Sex determination in northern painted turtles: effect of incubation at constant and fluctuating temperatures. Can J Zool 63:2543–2547Google Scholar
  33. Schwarzkopf L, Brooks RJ (1987) Nest-site selection and offspring sex ratio in painted turtles, Chrysemys picta. Copeia 1987:53–61Google Scholar
  34. Shine R, Harlow PS (1996) Maternal manipulation of offspring phenotypes via nest-site selection in an oviparous lizard. Ecology 77:1808–1818.Google Scholar
  35. Sokal RR, Rohlf FJ (1995) Biometry. Freeman, New YorkGoogle Scholar
  36. Thomas RB, Vogrin N, Altig R (1999) Sexual and seasonal differences in behavior of Trachemys scripta (Testudines: Emydidae). J Herpetol 33:511–515Google Scholar
  37. Valenzuela N, Janzen FJ (2001) Nest-site philopatry and the evolution of temperature-dependent sex determination. Evol Ecol Res 3:1–17Google Scholar
  38. Vogt RC, Bull JJ (1984) Ecology of hatchling sex ratio in map turtles. Ecology 65:582–587Google Scholar
  39. Vogt RC, Flores-Villela O (1992) Effects of incubation temperature on sex determination in a community of neotropical freshwater turtles in southern Mexico. Herpetologica 48:265–270Google Scholar
  40. Wade M (1998) The evolutionary genetics of maternal effects. In: Mousseau TA, Fox CW (eds) Maternal effects as adaptations. Oxford University Press, New York, pp 5–21Google Scholar
  41. Weisrock DW, Janzen FJ (1999) Thermal and fitness-related consequences of nest location in painted turtles (Chrysemys picta). Funct Ecol 13:94–101CrossRefGoogle Scholar
  42. Wilhoft DC, Hotaling E, Franks P (1983) Effects of temperature on sex determination in embryos of the snapping turtle, Chelydra serpentina. J Herpetol 17:38–42Google Scholar
  43. Wilson DS (1998) Nest-site selection: microhabitat variation and its effects on the survival of turtle embryos. Ecology 79:1884–1892Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Department of Zoology and GeneticsIowa State UniversityAmesUSA
  2. 2.Department of BiologyIndiana UniversityUSA

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