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International Orthopaedics

, Volume 38, Issue 5, pp 1011–1017 | Cite as

Interference in the endplate nutritional pathway causes intervertebral disc degeneration in an immature porcine model

  • Ran Kang
  • Haisheng Li
  • Steffen Ringgaard
  • Kresten Rickers
  • Haolin Sun
  • Muwan Chen
  • Lin Xie
  • Cody Bünger
Original Paper

Abstract

Purpose

Previous studies have shown that blocking the endplate nutritional pathway with bone cement did not result in obvious intervertebral disc degeneration (IDD) in mature animal models. However, there are very few comparable studies in immature animal models. As vertebroplasty currently is beginning to be applied in young, even biologically immature patients, it is important to investigate the effect of cement blocking at the endplate in an immature animal model.

Methods

Two lumbar intervertebral discs in eight immature pigs were either blocked by cement in both endplate pathways or stabbed with a scalpel in the annulus fibrosus (AF) as a positive control, and with a third disc remaining intact as a normal control. Magnetic resonance imaging (MRI) and histology study were performed.

Results

After three months, the cement-blocked discs exhibited severe IDD, with the percentage of disc-height index (DHI), nucleus pulposus (NP) area, and NP T2 value significantly lower than the normal control. These IDD changes were histologically confirmed. Post-contrast MRI showed diseased nutritional diffusion patterns in the cement-blocked discs. Moreover, the degenerative changes of the cement-blocked discs exceeded those of the injured AF positive controls.

Conclusions

The endplate nutritional pathway was interfered with and diseased after three months of bone cement intervention in an immature porcine model. Severe interference in the endplate nutritional pathway in an immature porcine model caused IDD. These findings also draw attention to the fact that interference in endplate nutritional pathways in immature or young patients may affect the vitality of adjacent discs.

Keywords

Endplate nutritional pathway Intervertebral disc degeneration Vertebroplasty Bone cement MRI Immature porcine model 

Notes

Acknowledgements

We thank Anette Baatrup, Dandan Chen, and Yufen Zhang for their technical and surgical assistance. We gratefully acknowledge the funding from Velux (25906), Lundbeck, Gigtforeningen Foundation, and the International Cooperation and Natural Science Foundation of Jiansu province of China (BZ2011046, BK2012490).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ran Kang
    • 1
    • 3
  • Haisheng Li
    • 1
  • Steffen Ringgaard
    • 2
  • Kresten Rickers
    • 1
  • Haolin Sun
    • 1
  • Muwan Chen
    • 1
  • Lin Xie
    • 3
  • Cody Bünger
    • 1
  1. 1.Orthopaedic Research LaboratoryAarhus UniversityAarhus CDenmark
  2. 2.The MR Research Centre, Aarhus University HospitalAarhus CDenmark
  3. 3.Department of Orthopedic SurgeryJiangsu Province Hospital on Integration of Chinese and Western MedicineNanjingChina

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