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International Orthopaedics

, Volume 36, Issue 9, pp 1961–1966 | Cite as

Behaviour of human physeal chondro-progenitorcells in early growth plate injury response in vitro

  • Karin Pichler
  • Barbara Schmidt
  • Eva E. Fischerauer
  • Beate Rinner
  • Gottfried Dohr
  • Andreas Leithner
  • Annelie M. Weinberg
Original Paper

Abstract

Purpose

The aim of this study was to investigate the proliferation and differentiation behaviour of a defined cell population gained from the human growth plate, namely, chondro-progenitorcells (CPCs), in the initial inflammatory phase of growth plate injury response in vitro.

Methods

Growth plate cells were sorted via FACS and differentiated along adipogenic and osteogenic lineage to confirm their progenitor features. To mimic the inflammatory phase of injury response at the growth plate they were treated with IL-1β and exposed to cyclic mechanical loading. A BrdU assay was used to investigate CPC proliferation. CPC differentiation behaviour was analysed by RT-PCR.

Results

CPCs (CD45-, CD34-, CD73+, CD90+, and CD105+) showed a successful differentiation along adipogenic and osteogenic lineage. Under conditions simulating the inflammatory phase of injury response at the growth plate in vitro CPCs differentiated towards hypertrophy while chondrogenesis and ossification were inhibited. Proliferation was not significantly altered.

Conclusion

This study showed that CPCs can be isolated from the human growth plate and expanded in vitro. In the first phase of injury response at the growth plate these cells differentiate towards hypertrophy. As longitudinal growth is obtained by chondrocyte proliferation and volume increase during hypertrophy this maturation might be the first step towards post-traumatic growth disorders such as unwanted premature ossification of the growth plate.

Keywords

Growth Plate Injury Response Inflammatory Phase Bone Bridge Growth Plate Chondrocytes 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors appreciate support from the Laura Bassi Center of Expertise BRIC (Bioresorbable Implants for Children; FFG -Austria). Furthermore they would like to acknowledge Rudolf Schmied for his valuable technical assistance. They declare no actual or potential conflict of interest.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Karin Pichler
    • 1
  • Barbara Schmidt
    • 1
  • Eva E. Fischerauer
    • 1
  • Beate Rinner
    • 2
  • Gottfried Dohr
    • 3
  • Andreas Leithner
    • 4
  • Annelie M. Weinberg
    • 4
  1. 1.Department of Pediatric and Adolescent SurgeryMedical University GrazGrazAustria
  2. 2.Center for Medical Research – ZMFMedical University GrazGrazAustria
  3. 3.Institute of Cell Biology, Histology and Embryology, Center for Molecular MedicineMedical University of GrazGrazAustria
  4. 4.Department of Orthopaedic SurgeryMedical University of GrazGrazAustria

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