International Orthopaedics

, Volume 30, Issue 5, pp 357–361

Rabbit articular cartilage defects treated by allogenic chondrocyte transplantation

  • P. R. J. V. C. Boopalan
  • Solomon Sathishkumar
  • Senthil Kumar
  • Samuel Chittaranjan
Original Paper

Abstract

Articular cartilage defects have a poor capacity for repair. Most of the current treatment options result in the formation of fibro-cartilage, which is functionally inferior to normal hyaline articular cartilage. We studied the effectiveness of allogenic chondrocyte transplantation for focal articular cartilage defects in rabbits. Chondrocytes were cultured in vitro from cartilage harvested from the knee joints of a New Zealand White rabbit. A 3 mm defect was created in the articular cartilage of both knees in other rabbits. The cultured allogenic chondrocytes were transplanted into the defect in the right knees and closed with a periosteal flap, while the defects in the left knees served as controls and were closed with a periosteal flap alone, without chondrocytes. Healing of the defects was assessed at 12 weeks by histological studies. Allogenic chondrocyte transplantation significantly increased the amount of newly formed repair tissue (P=0.04) compared with that found in the control knees. The histological quality score of the repair tissue was significantly better (P=0.05), with more hyaline characteristics in the knees treated with allogenic chondrocytes than in the control knees. Articular cartilage defects treated with allogenic chondrocyte transplantation result in better repair tissue formation with hyaline characteristics than those in control knees.

Résumé

Nous avons étudié l’efficacité de la transplantation de chondrocytes allogènes pour des pertes de substances localisées chez le lapin. Une perte de substance de 3 mm a été crée au niveau des 2 genoux. Des chondrocytes ont été cultivés in vitro à partir de prélèvements fait sur les genoux de lapin blanc de Nouvelle Zélande. Les chondrocytes cultivés ont été transplantés dans le defect du genou droit et enfermés sous un lambeau périosté tandis que le genou gauche servait de contrôle avec un lambeau périosté sans chondrocyte. La cicatrisation cartilagineuse a été étudié histologiquement à 12 semaines. La transplantation de chondrocytes allogènes augmente significativement la quantité de tissu de réparation en comparant aux genoux de contrôle (p=0.04). Le score histologique est meilleur que dans le groupe de contrôle.

References

  1. 1.
    Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331:889–895PubMedCrossRefGoogle Scholar
  2. 2.
    Brittberg M, Nilsson A, Lindahl A, Ohlsson C, Peterson L (1996) Rabbit articular cartilage defects treated with autologous cultured chondrocytes. Clin Orthop 326:270–283CrossRefPubMedGoogle Scholar
  3. 3.
    Brittberg M, Lars Peterson, Eva Sjouml, Tommi Tallheden, Anders Lindahl (2003) Articular cartilage engineering with autologous chondrocyte transplantation. A review of recent developments. J Bone Joint Surg 85A:109–115Google Scholar
  4. 4.
    Buckwalter JA, Mankin HJ (1999) Articular cartilage. Part II: degeneration and osteoarthrosis, repair, regeneration, and transplantation. J Bone Joint Surg 79A:612–632Google Scholar
  5. 5.
    Chesterman PJ, Smith AU (1968) Homotransplantation of articular cartilage and isolated chondrocytes: an experimental study in rabbits. J Bone Joint Surg 50B:184Google Scholar
  6. 6.
    Coletti JM Jr, Akeson WH, Woo SL-Y (1972) A comparison of the physical behaviour of normal articular cartilage and the arthroplasty surface. J Bone Joint Surg 54A:147–160Google Scholar
  7. 7.
    Elves MW (1974) A study of transplantation antigens on chondrocytes from articular cartilage. J Bone Joint Surg 56B:178Google Scholar
  8. 8.
    Engkvist O, Ohlsen L (1979) Reconstruction of articular cartilage with free autologous perichondrial grafts: an experimental study in rabbits. Scand J Plast Reconstr Surg 13:269–274PubMedGoogle Scholar
  9. 9.
    Ficat RP, Ficat C, Gedeon P, Toussaint JB (1979) Spongialization: a new treatment for diseased patellae. Clin Orthop 144:74–83PubMedGoogle Scholar
  10. 10.
    Furukawa T, Eyre DR, Koide DR (1980) Biochemical studies on repair cartilage resurfacing experimental defects in the rabbit knee. J Bone Joint Surg 62A:79–89Google Scholar
  11. 11.
    Horas U, Pelinkovic D, Herr G, Aigner T, Schnettler R (2003) Autologous chondrocyte implantation and osteochondral cylinder transplantation in cartilage repair of the knee joint. A prospective, comparative trial. J Bone Joint Surg 85A:185–192Google Scholar
  12. 12.
    Hubbard MJ (1996) Articular debridement versus washout for degeneration of the medial femoral condyle. A five-year study. J Bone Joint Surg 78B:217–219Google Scholar
  13. 13.
    Insall J (1974) The Pridie debridement operation for osteoarthritis of the knee. Clin Orthop 101:61–67PubMedGoogle Scholar
  14. 14.
    Johnson-Nurse C, Dandy DJ (1985) Fracture-separation of articular cartilage in the adult knee. J Bone Joint Surg 67B:42–43Google Scholar
  15. 15.
    Kawabe N, Yoshinao M (1991) The repair of full-thickness articular cartilage defects. Immune responses to reparative tissue formed by allogenic growth plate chondrocyte implants. Clin Orthop 268:279–293Google Scholar
  16. 16.
    Knutsen G, Engebretsen L, Ludvigsen TC, Drogset JO, Grontvedt T, Solheim E, Strand T, Roberts S, Isaksen V, Johansen O (2004) Autologous chondrocyte implantation compared with microfracture in the knee. A randomized trial. J Bone Joint Surg 86A:455–464Google Scholar
  17. 17.
    Mckibbin B (1971) Immature joint cartilage and the homograft reaction. J Bone Joint Surg 53B:123Google Scholar
  18. 18.
    Mitchell N, Shepard N (1976) The resurfacing of adult rabbit articular cartilage by multiple perforations through the subchondral bone. J Bone and Joint Surg 58A:230–233Google Scholar
  19. 19.
    Moseley JB, O’Malley K, Petersen NJ, Menke TJ, Brody BA, Kuykendall DH, Hollingsworth JC, Ashton CM, Wray NP (2002) A controlled trial of arthroscopic surgery for osteoarthritis of the knee. N Engl J Med 347:81–88CrossRefPubMedGoogle Scholar
  20. 20.
    Noguchi T, Oka M, Fujino M, Neo M, Yamamuro T (1994) Repair of osteochondral defects with grafts of cultured chondrocytes. Comparison of allografts and isografts. Clin Orthop 302:251–258PubMedGoogle Scholar
  21. 21.
    Peterson L, Minas T, Brittberg M, Nilsson A, Sjogren-Jansson E, Lindall A (2000) Two- to 9-year outcome after autologous chondrocyte transplantation of the knee. Clin Orthop 374:212–234CrossRefPubMedGoogle Scholar
  22. 22.
    Pineda S, Pollack A, Stevenson S (1992) A semiquantitative scale for histologic grading of articular cartilage repair. Acta Anat 143:335–340PubMedCrossRefGoogle Scholar
  23. 23.
    Rothwell AG (1990) Synovium transplantation onto the cartilage denuded patellar groove of the sheep knee joint. Orthopedics 13:433–442PubMedGoogle Scholar
  24. 24.
    Rubak JM, Poussa M, Ritsila V (1982) Chondrogenesis in repair of articular cartilage defects by free periosteal grafts in rabbits. Acta Orthop Scand 53:181–186PubMedCrossRefGoogle Scholar
  25. 25.
    Wasiak JVE (ed) (2005) Autologous cartilage implantation for full thickness articular cartilage defects of the knee (Cochrane Review). The Cochrane Library, Wiley, Chichester, UKGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • P. R. J. V. C. Boopalan
    • 1
  • Solomon Sathishkumar
    • 2
  • Senthil Kumar
    • 3
  • Samuel Chittaranjan
    • 1
  1. 1.Department Of OrthopaedicsChristian Medical CollegeVelloreIndia
  2. 2.Department Of PhysiologyChristian Medical CollegeVelloreIndia
  3. 3.Central Animal Housing FacilityChristian Medical CollegeVelloreIndia

Personalised recommendations