Cancer Immunology, Immunotherapy

, Volume 50, Issue 6, pp 328–333 | Cite as

Interleukin-10 in serous ovarian carcinoma cell lines

  • Stefan Berger
  • Antje Siegert
  • Carsten Denkert
  • Martin Köbel
  • Steffen Hauptmann
ORIGINAL ARTICLE

Abstract

 Interleukin-10, one of the most potent anti-inflammatory cytokines, is expressed in ovarian carcinomas in vivo. In contrast to the high levels of IL-10 in ascites and tumour tissue, the expression of this cytokine appears to be a rare event in ovarian carcinoma cell lines in vitro. Virtually nothing is known about the regulation of IL-10 expression in ovarian carcinoma cell lines. We investigated the expression of IL-10 in four cell lines originally derived from ovarian serous adenocarcinoma: OVCAR-3, SKOV-3, CAOV-3 and OAW-42. IL-10-specific mRNA was detected in OVCAR-3 and only this cell line produced IL-10 constitutively under serum-free conditions as well as in serum-containing medium. Our studies on the regulation of IL-10 secretion in OVCAR-3 revealed that (1) proinflammatory stimuli IL-1β and TNF-α, but not LPS, enhance IL-10 secretion, (2) IL-6 has no influence on the release of IL-10, (3) prostaglandin E2 influences neither the spontaneous nor the TNF-α- or IL-1β-stimulated IL-10 production and (4) interferon-γ inhibits IL-10 secretion. We conclude that only a minority of serous ovarian carcinoma cells maintain the ability to produce IL-10 in vitro. Our data on the regulation of IL-10 production in OVCAR-3 indicate that ovarian carcinoma cells share some, but not all, of the regulatory features typical for the monocytic IL-10 secretion.

Key words Interleukin-10 Ovarian cancer 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Stefan Berger
    • 1
  • Antje Siegert
    • 1
  • Carsten Denkert
    • 1
  • Martin Köbel
    • 1
  • Steffen Hauptmann
    • 1
  1. 1.Institute of Pathology, Charité Hospital Berlin, Schumannstrasse 20/21, 10117 Berlin, Germany e-mail: stefan.berger@charite.de Tel.:  +49-30-28023420; Fax:  +49-30-28021296DE

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